TY - GEN
T1 - In Vitro Artery Model with Circumferentially Aligned & Contractible Smooth Muscle by Unfixed Molding & Screwing Fabrication
AU - Itai, Shun
AU - Onoe, Hiroaki
N1 - Funding Information:
This work was partly supported by JSPS KAKENHI Grant Number JP YYK0F12, Japan Society for the Promotion of Science (JSPS), Japan, and Fukuzawa Foundation Research Grant, Keio University, Japan
Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - This paper describes a fabrication method of an artery model with circumferentially aligned and contractible smooth muscle cells (SMCs) by unfixed molding and screwing processes. The fabricated aligned smooth muscle is well-deformable, enabling the flexible contraction in biological reaction. The axially aligned fiber-shaped smooth muscle tissue was formed by unfixed molding and screwed up to be aligned circumferentially. Also, the SMC layer was covered with a collagen layer with connectors to enable co-culture with endothelial cells (ECs) and perfusion culture. The alignment of SMCs was visibly confirmed, and the ability to reproduce the biochemical contraction was proved by an inflammatory reaction. We believe that our novel fabrication method would contribute to the development of functional smooth muscle models for biomedical researches.
AB - This paper describes a fabrication method of an artery model with circumferentially aligned and contractible smooth muscle cells (SMCs) by unfixed molding and screwing processes. The fabricated aligned smooth muscle is well-deformable, enabling the flexible contraction in biological reaction. The axially aligned fiber-shaped smooth muscle tissue was formed by unfixed molding and screwed up to be aligned circumferentially. Also, the SMC layer was covered with a collagen layer with connectors to enable co-culture with endothelial cells (ECs) and perfusion culture. The alignment of SMCs was visibly confirmed, and the ability to reproduce the biochemical contraction was proved by an inflammatory reaction. We believe that our novel fabrication method would contribute to the development of functional smooth muscle models for biomedical researches.
KW - Alignment
KW - In vitro blood vessel
KW - Molding
KW - Smooth Muscle Cells
KW - Tissue engineering
KW - collagen
KW - histamine
UR - http://www.scopus.com/inward/record.url?scp=85126394176&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85126394176&partnerID=8YFLogxK
U2 - 10.1109/MEMS51670.2022.9699803
DO - 10.1109/MEMS51670.2022.9699803
M3 - Conference contribution
AN - SCOPUS:85126394176
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 275
EP - 278
BT - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Y2 - 9 January 2022 through 13 January 2022
ER -