In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)


Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Adoptive T cell therapy
  • Cancer immunotherapy
  • Coculture with feeder cells
  • Notch signaling
  • Stem cell memory T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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