TY - CHAP
T1 - In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells
AU - Kondo, Taisuke
AU - Imura, Yuki
AU - Ando, Makoto
AU - Chikuma, Shunsuke
AU - Yoshimura, Akihiko
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.
AB - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.
KW - Adoptive T cell therapy
KW - Cancer immunotherapy
KW - Coculture with feeder cells
KW - Notch signaling
KW - Stem cell memory T cells
UR - http://www.scopus.com/inward/record.url?scp=85071280427&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071280427&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9728-2_4
DO - 10.1007/978-1-4939-9728-2_4
M3 - Chapter
C2 - 31396927
AN - SCOPUS:85071280427
T3 - Methods in Molecular Biology
SP - 41
EP - 51
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
