In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo; Yuki Imura; Makoto Ando; Shunsuke Chikuma; Akihiko Yoshimura

doi:10.1007/978-1-4939-9728-2_4

In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Taisuke Kondo
, Yuki Imura
, Makoto Ando
, Shunsuke Chikuma
, Akihiko Yoshimura

Department of Microbiology and Immunology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Citations (Scopus)

Abstract

Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (T_SCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically T_SCM cells (iT_SCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iT_SCM cells, including reagents, culture setting, and procedure.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-51
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-9728-2_4
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	2048
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Adoptive T cell therapy
Cancer immunotherapy
Coculture with feeder cells
Notch signaling
Stem cell memory T cells

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-9728-2_4

Cite this

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abstract = "Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.",

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T1 - In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

AU - Kondo, Taisuke

AU - Imura, Yuki

AU - Ando, Makoto

AU - Chikuma, Shunsuke

AU - Yoshimura, Akihiko

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2019.

PY - 2019

Y1 - 2019

N2 - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

AB - Adoptive T cell therapy is an attractive strategy in tumor immunotherapy. The transfer of in vitro expanded tumor-associated antigen (TAA)-specific T cells from patients may effectively destroy the original tumor cells. One of the limitations is a rapid acquisition of tolerant (anergy, deletion, dysfunctional, and/or exhausted) phenotypes. We and others found that stem cell memory T (TSCM) cells are strongly resistant to tolerance, showing strong expansion and persistence in vivo and providing long-lasting antitumor effects. We previously established that phenotypically TSCM cells (iTSCM) can be induced using a simple coculture of activated T cells with OP9 stroma cells expressing a Notch ligand. Here, we describe a defined protocol for generating human iTSCM cells, including reagents, culture setting, and procedure.

KW - Adoptive T cell therapy

KW - Cancer immunotherapy

KW - Coculture with feeder cells

KW - Notch signaling

KW - Stem cell memory T cells

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UR - https://www.scopus.com/pages/publications/85071280427#tab=citedBy

U2 - 10.1007/978-1-4939-9728-2_4

DO - 10.1007/978-1-4939-9728-2_4

M3 - Chapter

C2 - 31396927

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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