In vitro effects of angiopoietins and VEGF on hematopoietic and endothelial cells

Xu Ling Huang, Nobuyuki Takakura, Toshio Suda

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


To investigate the role of TIE-2/angiopoietins (Angs) in postnatal hematopoiesis, we cultured bone marrow cells in the presence of Ang-1 and -2 with or without VEGF. While either Ang-1 or Ang-2 alone had hematopoietic effects on unseparated bone marrow cells and no effect on proliferation of endothelial cells, both enhanced the growth of endothelial cells and hematopoietic progenitor cells in the presence of VEGF. FACS analysis showed that Lin-TIE-2+Flk-1+ cells cocultured with OP9 stromal cells gave rise to endothelial and hematopoietic cells in the presence of VEGF and Ang-1. Ang-1 promoted the adhesion of sorted primary Lin-TIE-2+ cells to fibronectin, and this adhesion enhanced proliferation of hematopoietic progenitor cells synergistically with stem cell factor (SCF). Our findings suggest that TIE-2/angiopoietins may act as critical regulators of proliferation of hematopoietic progenitors and endothelial cells in a synergistic manner.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 1999 Oct 14
Externally publishedYes


  • Cell adhesion and proliferation
  • Endothelial cells
  • Hematopoietic progenitors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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