In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating

Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Kuniaki Nakanishi; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.530815

In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Kuniaki Nakanishi, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

Research output: Contribution to journal › Conference article › peer-review

Abstract

We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

Original language	English
Pages (from-to)	136-139
Number of pages	4
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5322
DOIs	https://doi.org/10.1117/12.530815
Publication status	Published - 2004 Oct 27
Event	Progress in Biomedical Optics and Imaging - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II - San Jose, CA, United States Duration: 2004 Jan 27 → 2004 Jan 28

Keywords

Gene therapy
Heat
Laser
Membrane fluidity
Stress wave

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.530815

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abstract = "We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.",

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T2 - Progress in Biomedical Optics and Imaging - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

AU - Terakawa, Mitsuhiro

AU - Sato, Shunichi

AU - Ogura, Makoto

AU - Nakanishi, Kuniaki

AU - Masaki, Yoshinori

AU - Uenoyama, Maki

AU - Wakisaka, Hitoshi

AU - Ashida, Hiroshi

AU - Obara, Minoru

PY - 2004/10/27

Y1 - 2004/10/27

N2 - We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

AB - We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (∼43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

KW - Gene therapy

KW - Heat

KW - Laser

KW - Membrane fluidity

KW - Stress wave

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In vitro gene transfer by the application of laser-induced stress wave: Effect of cellular heating

Abstract

Keywords

ASJC Scopus subject areas

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