In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus

Neeta Singh Roy, Su Wang, Li Jiang, Jian Kang, Abdellatif Benraiss, Catherine Harrison-Restelli, Richard A.R. Fraser, William T. Couldwell, Ayano Kawaguchi, Hideyuki Okano, Maiken Nedergaard, Steven A. Goldman

Research output: Contribution to journalArticlepeer-review

497 Citations (Scopus)


Neurogenesis persists in the adult mammalian hippocampus. To identify and isolate neuronal progenitor cells of the adult human hippocampus, we transfected ventricular zone-free dissociates of surgically-excised dentate gyrus with DNA encoding humanized green fluorescent protein (hGFP), placed under the control of either the nestin enhancer (E/nestin) or the Tα1 tubulin promoter (P/Tα1), two regulatory regions that direct transcription in neural progenitor cells. The resultant P/Tα1:hGFP+, and E/nestin:enhanced (E)GFP+ cells expressed βIII-tubulin or microtubule- associated protein-2; many incorporated bromodeoxyuridine, indicating their genesis in vitro. Using fluorescence-activated cell sorting, the E/nestin:EGFP+ and P/Tα1:hGFP+ cells were isolated to near purity, and matured antigenically and physiologically as neurons. Thus, the adult human hippocampus contains mitotically competent neuronal progenitors that can be selectively extracted. The isolation of these cells may provide a cellular substrate for re-populating the damaged or degenerated adult hippocampus.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalNature medicine
Issue number3
Publication statusPublished - 2000 Mar
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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