In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.

Akira Ishizuka; Kuniaki Saito; Mikiko C. Siomi; Haruhiko Siomi

In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.

Akira Ishizuka
, Kuniaki Saito
, Mikiko C. Siomi
, Haruhiko Siomi

Research output: Contribution to journal › Review article › peer-review

Abstract

Recent studies have shown that the microRNA (miRNA) pathway is an evolutionarily conserved endogenous pathway that is important for normal development. Mature miRNAs are excised from precursors in a stepwise process and subsequently incorporated into an RNA-induced silencing complex (RISC), which mediates either cleavage of the target messenger RNA (mRNA) or translational repression, depending on the complementarity between the miRNA and its target mRNA. In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials.

Original language	English
Pages (from-to)	277-286
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	342
Publication status	Published - 2006
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

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abstract = "Recent studies have shown that the microRNA (miRNA) pathway is an evolutionarily conserved endogenous pathway that is important for normal development. Mature miRNAs are excised from precursors in a stepwise process and subsequently incorporated into an RNA-induced silencing complex (RISC), which mediates either cleavage of the target messenger RNA (mRNA) or translational repression, depending on the complementarity between the miRNA and its target mRNA. In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials.",

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AU - Saito, Kuniaki

AU - Siomi, Mikiko C.

AU - Siomi, Haruhiko

PY - 2006

Y1 - 2006

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AB - Recent studies have shown that the microRNA (miRNA) pathway is an evolutionarily conserved endogenous pathway that is important for normal development. Mature miRNAs are excised from precursors in a stepwise process and subsequently incorporated into an RNA-induced silencing complex (RISC), which mediates either cleavage of the target messenger RNA (mRNA) or translational repression, depending on the complementarity between the miRNA and its target mRNA. In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials.

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M3 - Review article

C2 - 16957382

AN - SCOPUS:33749077732

SN - 1064-3745

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SP - 277

EP - 286

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.

Abstract

ASJC Scopus subject areas

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