In vivo hypoglycemic effects, potential mechanisms and LC-MS/MS analysis of dendropanax Trifidus sap extract

Ahreum Lee, Yuki Sugiura, Ik Hyun Cho, Noriko Setou, Eugene Koh, Gyun Jee Song, Seungheun Lee, Hyun Jeong Yang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Extracts of medicinal plants have been widely used to benefit human health. Dendropanax morbiferus (DM) has been well-studied for its anti-inflammatory and anti-oxidative effects, while Dendropanax trifidus (DT) is a lesser-known ecotype phylogenetically similar to DM, which has received significantly less attention. Studies thus far have primarily focused on leaf and bark extracts of DM, and not much is yet known about the properties of either DM or DT sap. Therefore, here we performed in vivo toxicity and efficacy studies, in order to assess the biological effects of DT sap. To establish a safe dosage range, single dose or two-week daily administrations of various concentrations were performed for ICR mice. Measurements of survival ratio, body/organ weight, blood chemistry, histochemistry and Western blots were performed. A concentration of ≤0.5 mg/g DT sap was found to be safe for long-term administration. Interestingly, DT sap significantly reduced blood glucose in female mice. In addition, increasing concentrations of DT sap decreased phosphorylated (p) insulin receptor substrate (IRS)-1(ser1101)/IRS-1 in liver tissues, while increasing pAMP-activated protein kinase (AMPK)/AMPK in both the liver and spleen. To analyze its components, liquid chromatography-tandem mass spectrometry of DT sap was performed in comparison with Acer sac-charum (AS) sap. Components such as estradiol, trenbolone, farnesol, dienogest, 2-hydroxyestradiol and linoleic acid were found to be highly enriched in DT sap compared to AS sap. Our results indicate DT sap exhibits hypoglycemic effects, which may be due to the abundance of the bioactive components.

Original languageEnglish
Article number4332
Issue number12
Publication statusPublished - 2021 Dec


  • AMPK
  • Blood glucose
  • Dendropanax morbiferus
  • Dendropanax trifidus
  • In vivo toxicity
  • LC-MS/MS

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


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