In Vivo Tracing of Neural Tracts in Tip-Toe Walking Yoshimura Mice by Diffusion Tensor Tractography

Tsunehiko Konomi, Morito Takano, Yuji Komaki, Keigo Hikishima, Kanehiro Fujiyoshi, Osahiko Tsuji, Hideyuki Okano, Morio Matsumoto, Masaya Nakamura

Research output: Chapter in Book/Report/Conference proceedingChapter


In ossification of the posterior longitudinal ligaments (OPLL), axonal disruption results in motor and sensory function impairment. Twy (tip-toe walking Yoshimura) mice develop spontaneous calcification in the cervical ligaments, thereby causing chronic compression of the spinal cords. To determine whether in vivo DTT can evaluate the axonal disruption of the chronic compressive spinal cords in twy mice, 6-, 15-, and 20-week-old twy mice were chronologically subjected to DTT. MRI was performed using a 7.0-T magnet (Biospec 70/16) with CryoProbe (Bruker biospin GmbH, Ettlingen, Germany). Diffusion tensor images were analyzed using TrackVis (Massachusetts General Hospital, MA, USA). We succeeded in depicting in vivo high-resolution DTT of the twy mice. The progress of the ligamentous calcification was observed at C2-3 level in each twy mouse, and the number of RT-97- or SMI-31-positive fibers was decreased depending on the severity of the compression of the spinal cord. We demonstrated that quantitative analysis of sequential DTT enabled to detect subtle damage of the compressed spinal cord prior to the deterioration of neurological function in twy mice. Thus, in a clinical setting, DTT could be a new effective imaging modality in patients with cervical OPLL.

Original languageEnglish
Title of host publicationOPLL
Subtitle of host publicationOssification of the Posterior Longitudinal Ligament: Third Edition
PublisherSpringer Singapore
Number of pages8
ISBN (Electronic)9789811538551
ISBN (Print)9789811538544
Publication statusPublished - 2020 Jan 1


  • Cervical myelopathy
  • Diffusion tensor tractography
  • Digigait analysis
  • Ossification of the posterior longitudinal ligament
  • Rotarod treadmill
  • RT-97
  • SMI-31
  • Spinal cord compression
  • Stejskal-Tanner diffusion preparation
  • Tract fiber
  • twy mouse

ASJC Scopus subject areas

  • Medicine(all)


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