TY - JOUR
T1 - In Vivo visualization of oxygen radical-dependent photoemission
AU - Tsuchiya, Masaharu
AU - Suematsu, Makoto
AU - Suzuki, Hidekazu
N1 - Funding Information:
The authors thank Professor Yuzuru Ishimura of the Department of Biochemistry, Keio University, Professor Takeshi Nishino of the First Department of Biochemistry, Nippon Medical University, and Professor Geert W. Schmid-Sch6nbein and Professor Emeritus Benjamin W. Zweifach of the Institute for Biomedical Engineering, University of California, San Diego, for fruitful suggestions. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and by a grant from Keio University School of Medicine.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - Oxygen radicals are mediators of tissue injury in a wide variety of pathophysiological conditions. These active oxygen metabolites can interact with cellular membrane structures, thereby inducing lipid peroxidation, which has been implicated as one of the major mechanisms of “oxidative stress” in tissue injury. This chapter presents evidence illustrating topographic correlation between oxidative changes and distribution of cell injury. It remains unclear whether in-site oxidant production may play a causal role in the same portion of cell injury, or whether malondialdehyde elevation or decrease in reduced glutathione contents in tissue may be merely a consequence of tissue injury that may result from oxidant-independent mechanisms. The chapter discusses current methodology to monitor visually spatial and temporal alterations of oxidative changes in addition to monitoring simultaneous cell function and viability in in vivo or ex vivo organ microcirculation. The chemiluminescence imaging method has several properties that prove useful for evaluation of oxidative stress in vivo: (1) the absence of an artificial photobleaching effect, (2) no need for excitation illumination, resulting in attenuation of cellular damages, and (3) the availability of different chemilumigenic probes, which makes it possible to pinpoint the active oxidants that are involved in different experimental models.
AB - Oxygen radicals are mediators of tissue injury in a wide variety of pathophysiological conditions. These active oxygen metabolites can interact with cellular membrane structures, thereby inducing lipid peroxidation, which has been implicated as one of the major mechanisms of “oxidative stress” in tissue injury. This chapter presents evidence illustrating topographic correlation between oxidative changes and distribution of cell injury. It remains unclear whether in-site oxidant production may play a causal role in the same portion of cell injury, or whether malondialdehyde elevation or decrease in reduced glutathione contents in tissue may be merely a consequence of tissue injury that may result from oxidant-independent mechanisms. The chapter discusses current methodology to monitor visually spatial and temporal alterations of oxidative changes in addition to monitoring simultaneous cell function and viability in in vivo or ex vivo organ microcirculation. The chemiluminescence imaging method has several properties that prove useful for evaluation of oxidative stress in vivo: (1) the absence of an artificial photobleaching effect, (2) no need for excitation illumination, resulting in attenuation of cellular damages, and (3) the availability of different chemilumigenic probes, which makes it possible to pinpoint the active oxidants that are involved in different experimental models.
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U2 - 10.1016/S0076-6879(94)33015-8
DO - 10.1016/S0076-6879(94)33015-8
M3 - Article
C2 - 8015451
AN - SCOPUS:0028233552
SN - 0076-6879
VL - 233
SP - 128
EP - 140
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -