TY - JOUR
T1 - Induction of heme-oxygenase-1 (HO-1) does not enhance adiponectin production in human adipocytes
T2 - Evidence against a direct HO-1 - Adiponectin axis
AU - Yang, Mengliu
AU - Kimura, Masaki
AU - Ng, Choaping
AU - He, Jingjing
AU - Keshvari, Sahar
AU - Rose, Felicity J.
AU - Barclay, Johanna L.
AU - Whitehead, Jonathan P.
N1 - Funding Information:
This work was funded by a University of Queensland International Research Scholarship to MY and an Australian National Health and Medical Research Council Fellowship to JPW ( 511104 ).
Publisher Copyright:
© 2015 Elsevier Ireland Ltd.
PY - 2015/9/5
Y1 - 2015/9/5
N2 - Adiponectin is a salutary adipokine and hypoadiponectinemia is implicated in the aetiology of obesity-related inflammation and cardiometabolic disease making therapeutic strategies to increase adiponectin attractive. Emerging evidence, predominantly from preclinical studies, suggests induction of heme-oxygenase-1 (HO-1) increases adiponectin production and reduces inflammatory tone. Here, we aimed to test whether induction of HO-1 enhanced adiponectin production from mature adipocytes. Treatment of human adipocytes with cobalt protoporphyrin (CoPP) or hemin for 24-48 h increased HO-1 expression and activity without affecting adiponectin expression and secretion. Treatment of adipocytes with TNFα reduced adiponectin secretion and increased expression and secretion of additional pro-inflammatory cytokines, IL-6 and MCP-1, as well as expression of sXBP-1, a marker of ER stress. HO-1 induction failed to reverse these effects. These results demonstrate that induction of HO-1 does not directly enhance adiponectin production or ameliorate the pro-inflammatory effects of TNFα and argue against a direct HO-1 - adiponectin axis.
AB - Adiponectin is a salutary adipokine and hypoadiponectinemia is implicated in the aetiology of obesity-related inflammation and cardiometabolic disease making therapeutic strategies to increase adiponectin attractive. Emerging evidence, predominantly from preclinical studies, suggests induction of heme-oxygenase-1 (HO-1) increases adiponectin production and reduces inflammatory tone. Here, we aimed to test whether induction of HO-1 enhanced adiponectin production from mature adipocytes. Treatment of human adipocytes with cobalt protoporphyrin (CoPP) or hemin for 24-48 h increased HO-1 expression and activity without affecting adiponectin expression and secretion. Treatment of adipocytes with TNFα reduced adiponectin secretion and increased expression and secretion of additional pro-inflammatory cytokines, IL-6 and MCP-1, as well as expression of sXBP-1, a marker of ER stress. HO-1 induction failed to reverse these effects. These results demonstrate that induction of HO-1 does not directly enhance adiponectin production or ameliorate the pro-inflammatory effects of TNFα and argue against a direct HO-1 - adiponectin axis.
KW - Adiponectin
KW - HO-1
KW - Inflammation
KW - Therapeutic
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U2 - 10.1016/j.mce.2015.06.034
DO - 10.1016/j.mce.2015.06.034
M3 - Article
C2 - 26143632
AN - SCOPUS:84938200898
SN - 0303-7207
VL - 413
SP - 209
EP - 216
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
ER -
