TY - JOUR
T1 - Induction of Th17 cells by segmented filamentous bacteria in the murine intestine
AU - Farkas, Adam M.
AU - Panea, Casandra
AU - Goto, Yoshiyuki
AU - Nakato, Gaku
AU - Galan-Diez, Marta
AU - Narushima, Seiko
AU - Honda, Kenya
AU - Ivanov, Ivaylo I.
N1 - Funding Information:
This work was supported by the National Institutes of Health R01-DK098378 to I.I.I. and by the Crohn's and Colitis Foundation of America SRA#259540 to I.I.I. I.I.I. is a Pew Scholar in the Biomedical Sciences, supported by the Pew Charitable Trust.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/5/29
Y1 - 2015/5/29
N2 - Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.
AB - Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.
KW - CD4 T cells
KW - Commensal microbiota
KW - Intestinal epithelial cells
KW - Mucosa-associated bacteria
KW - SFB
KW - Segmented filamentous bacteria
KW - Th17 cells
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U2 - 10.1016/j.jim.2015.03.020
DO - 10.1016/j.jim.2015.03.020
M3 - Article
C2 - 25858227
AN - SCOPUS:84929964535
SN - 0022-1759
VL - 421
SP - 104
EP - 111
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -