Induction of Th17 cells by segmented filamentous bacteria in the murine intestine

Adam M. Farkas, Casandra Panea, Yoshiyuki Goto, Gaku Nakato, Marta Galan-Diez, Seiko Narushima, Kenya Honda, Ivaylo I. Ivanov

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)


Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalJournal of Immunological Methods
Publication statusPublished - 2015 May 29


  • CD4 T cells
  • Commensal microbiota
  • Intestinal epithelial cells
  • Mucosa-associated bacteria
  • SFB
  • Segmented filamentous bacteria
  • Th17 cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Induction of Th17 cells by segmented filamentous bacteria in the murine intestine'. Together they form a unique fingerprint.

Cite this