TY - GEN
T1 - Influence of biochemical composition on dynamic visco-elastic property of chondrocyte/agarose construct
AU - Miyata, Shogo
AU - Furukawa, Katsuko
AU - Ushida, Takashi
AU - Tateishi, Tetsuya
PY - 2005
Y1 - 2005
N2 - Recently, an approach to restore cartilage defects, culturing autologous chondrocytes to create a three dimensional tissue, has been developed. In this kind of approach, it is important to study whether the extracellular matrices synthesized by cultured chondrocytes have dynamic mechanical properties unique to articular cartilage. In this study, we evaluated in detail dynamic visco-elastic properties of chondrocyte/agarose constructs in compression and shear directions, and compared those properties with biochemical composition. As the results, the dynamic modulus of the constructs increased over the culture time. Furthermore, a regression analysis showed that the sulfated glycosaminoglycan (sGAG) content is correlated strongly with dynamic stiffness in both compression and shear deformation. On the other hand, loss tangent, which represents an energy dissipation capability of the constructs, showed little correlation with sGAG content.
AB - Recently, an approach to restore cartilage defects, culturing autologous chondrocytes to create a three dimensional tissue, has been developed. In this kind of approach, it is important to study whether the extracellular matrices synthesized by cultured chondrocytes have dynamic mechanical properties unique to articular cartilage. In this study, we evaluated in detail dynamic visco-elastic properties of chondrocyte/agarose constructs in compression and shear directions, and compared those properties with biochemical composition. As the results, the dynamic modulus of the constructs increased over the culture time. Furthermore, a regression analysis showed that the sulfated glycosaminoglycan (sGAG) content is correlated strongly with dynamic stiffness in both compression and shear deformation. On the other hand, loss tangent, which represents an energy dissipation capability of the constructs, showed little correlation with sGAG content.
KW - Articular cartilage
KW - Chondrocyte
KW - Dynamic visco-elasticity
KW - Mechanical property
KW - Tissue engineering
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M3 - Conference contribution
AN - SCOPUS:27844461251
SN - 0889864780
T3 - Proceedings of the 3rd IASTED International Conference on Biomedical Engineering 2005
SP - 515
EP - 518
BT - Proceedings of the 3rd IASTED International Conference on Biomedical Engineering 2005
A2 - Hamza, M.H.
T2 - 3rd IASTED International Conference on Medical Engineering 2005
Y2 - 16 February 2005 through 18 February 2005
ER -