Influence of gap junctions upon Ca2+ propagation from stimulated keratinocytes to DRG neurons

Chiaki Seto, Kenta Toyoda, Kousuke Inada, Kotaro Oka, Etsuro Ito

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Epidermal cells, such as keratinocytes, are regarded as the first sensory cells to transmit nociception and mechanoreception to free nerve endings extended from the dorsal root ganglion (DRG). Previous studies suggested that this transmission occurs as Ca2+ propagation via ATP receptors. Conversely, the influence of gap junctions on this Ca2+ propagation is largely unknown. Thus, we examined the localization and the role of connexin 43 among keratinocytes and DRG neurons. We co-cultured keratinocytes and DRG neurons and investigated the effect of pharmacological blockade of gap junctions on Ca2+ propagation upon stimulation of a single keratinocyte. Immunocytochemical experiments showed that connexin 43 is localized between keratinocytes and between keratinocytes and DRG neurons. Octanol, a gap junction inhibitor, significantly suppressed the concentrical Ca2+ propagation. Therefore, we conclude that the Ca2+ propagation mechanism via gap junctions from stimulated keratinocytes to free nerve endings should be taken into account.

Original languageEnglish
Article numbere190041
JournalBiophysics and physicobiology
Publication statusPublished - 2022


  • connexin 43
  • dorsal root ganglion
  • free nerve ending
  • octanol
  • skin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Physiology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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