Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase

Shoji Ohkuma, Sakae Shimizu, Masahiko Noto, Yoshimichi Sai, Kuninori Kinoshita, Hiro Omi Tamura

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 n M. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.

Original languageEnglish
Pages (from-to)862-866
Number of pages5
JournalIn Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Issue number11
Publication statusPublished - 1993 Nov
Externally publishedYes


  • NHCl
  • bafilomycin A
  • cell growth
  • lysosomal pH
  • transformation
  • vacuolar H-ATPase

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


Dive into the research topics of 'Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase'. Together they form a unique fingerprint.

Cite this