Inhibition of Phosphatidylinositol-specific Phospholipase C Activity by Fluvirucin B2

Hideaki Ui; Masaya Imoto; Kazuo Umezawa

doi:10.7164/antibiotics.48.387

Inhibition of Phosphatidylinositol-specific Phospholipase C Activity by Fluvirucin B₂

Hideaki Ui, Masaya Imoto, Kazuo Umezawa

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We isolated fluvirucin B₂from the culture broth of Streptomyces as an inhibitor of phosphatidylinositol-specific phospholipase C (PI-PLC). It inhibited PI-PLC of A431 cell cytosol with an IC₅₀ of 1.6μg/ml. Fluvirucin B₂also inhibited PI-PLC in cultured A431 cells, whereas it did not inhibit phosphatidylinositol synthesis and macromolecular synthesis markedly. It also inhibited epidermal growth factor-induced rapid rounding of A431 cells, in which PI turnover is involved.

Original language	English
Pages (from-to)	387-390
Number of pages	4
Journal	The Journal of Antibiotics
Volume	48
Issue number	5
DOIs	https://doi.org/10.7164/antibiotics.48.387
Publication status	Published - 1995 Jan 1

ASJC Scopus subject areas

Pharmacology
Drug Discovery

Access to Document

10.7164/antibiotics.48.387

Cite this

@article{1cf8189288494d2e8de7f670cfed0570,

title = "Inhibition of Phosphatidylinositol-specific Phospholipase C Activity by Fluvirucin B2",

abstract = "We isolated fluvirucin B2from the culture broth of Streptomyces as an inhibitor of phosphatidylinositol-specific phospholipase C (PI-PLC). It inhibited PI-PLC of A431 cell cytosol with an IC50 of 1.6μg/ml. Fluvirucin B2also inhibited PI-PLC in cultured A431 cells, whereas it did not inhibit phosphatidylinositol synthesis and macromolecular synthesis markedly. It also inhibited epidermal growth factor-induced rapid rounding of A431 cells, in which PI turnover is involved.",

author = "Hideaki Ui and Masaya Imoto and Kazuo Umezawa",

year = "1995",

month = jan,

day = "1",

doi = "10.7164/antibiotics.48.387",

language = "English",

volume = "48",

pages = "387--390",

journal = "The Journal of Antibiotics",

issn = "0021-8820",

publisher = "Japan Antibiotics Research Association",

number = "5",

}

TY - JOUR

T1 - Inhibition of Phosphatidylinositol-specific Phospholipase C Activity by Fluvirucin B2

AU - Ui, Hideaki

AU - Imoto, Masaya

AU - Umezawa, Kazuo

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We isolated fluvirucin B2from the culture broth of Streptomyces as an inhibitor of phosphatidylinositol-specific phospholipase C (PI-PLC). It inhibited PI-PLC of A431 cell cytosol with an IC50 of 1.6μg/ml. Fluvirucin B2also inhibited PI-PLC in cultured A431 cells, whereas it did not inhibit phosphatidylinositol synthesis and macromolecular synthesis markedly. It also inhibited epidermal growth factor-induced rapid rounding of A431 cells, in which PI turnover is involved.

AB - We isolated fluvirucin B2from the culture broth of Streptomyces as an inhibitor of phosphatidylinositol-specific phospholipase C (PI-PLC). It inhibited PI-PLC of A431 cell cytosol with an IC50 of 1.6μg/ml. Fluvirucin B2also inhibited PI-PLC in cultured A431 cells, whereas it did not inhibit phosphatidylinositol synthesis and macromolecular synthesis markedly. It also inhibited epidermal growth factor-induced rapid rounding of A431 cells, in which PI turnover is involved.

UR - http://www.scopus.com/inward/record.url?scp=0029045222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029045222&partnerID=8YFLogxK

U2 - 10.7164/antibiotics.48.387

DO - 10.7164/antibiotics.48.387

M3 - Article

C2 - 7797440

AN - SCOPUS:0029045222

SN - 0021-8820

VL - 48

SP - 387

EP - 390

JO - The Journal of Antibiotics

JF - The Journal of Antibiotics

IS - 5

ER -

Inhibition of Phosphatidylinositol-specific Phospholipase C Activity by Fluvirucin B₂

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this