Inhibition of Transcription by Mammalian Ribonucleic Acid Polymerase II: Effects of Diethylstilbestrol and Its Analogues

Taiko Oda; Yoshihiro Sato; Shigetaka Kitajima; Yukio Yasukochi

doi:10.1248/cpb.39.2627

Inhibition of Transcription by Mammalian Ribonucleic Acid Polymerase II: Effects of Diethylstilbestrol and Its Analogues

Taiko Oda, Yoshihiro Sato, Shigetaka Kitajima, Yukio Yasukochi

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We examined the effects of diethylstilbestrol (1), Z,Z-dienestrol (2), E,E-dienestrol (3), indenestrol A (4), indenestrol B (5) and estradiol (6) on the activity of in vitro accurate transcription from adenovirus 2 major late promoter by mammalian ribonucleic acid (RNA) polymerase II using partially purified transcription factors from HeLa cell nuclear extract as well as on RNA polymerase II activity assayed for random incorporation of ribonucleotides. 1, 2, and 3 inhibited both activities, whereas 4 and 5 were inhibitory only in the transcription activity at concentrations higher than 10/ig/ml. However 6 had no effects on both activities.

Original language	English
Pages (from-to)	2627-2629
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	39
Issue number	10
DOIs	https://doi.org/10.1248/cpb.39.2627
Publication status	Published - 1991 Jan 1

Keywords

RNA polymerase II
dienestrol
diethylstilbestrol
estradiol
indenestrol A
indenestrol B
transcription

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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10.1248/cpb.39.2627

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abstract = "We examined the effects of diethylstilbestrol (1), Z,Z-dienestrol (2), E,E-dienestrol (3), indenestrol A (4), indenestrol B (5) and estradiol (6) on the activity of in vitro accurate transcription from adenovirus 2 major late promoter by mammalian ribonucleic acid (RNA) polymerase II using partially purified transcription factors from HeLa cell nuclear extract as well as on RNA polymerase II activity assayed for random incorporation of ribonucleotides. 1, 2, and 3 inhibited both activities, whereas 4 and 5 were inhibitory only in the transcription activity at concentrations higher than 10/ig/ml. However 6 had no effects on both activities.",

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AU - Kitajima, Shigetaka

AU - Yasukochi, Yukio

PY - 1991/1/1

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N2 - We examined the effects of diethylstilbestrol (1), Z,Z-dienestrol (2), E,E-dienestrol (3), indenestrol A (4), indenestrol B (5) and estradiol (6) on the activity of in vitro accurate transcription from adenovirus 2 major late promoter by mammalian ribonucleic acid (RNA) polymerase II using partially purified transcription factors from HeLa cell nuclear extract as well as on RNA polymerase II activity assayed for random incorporation of ribonucleotides. 1, 2, and 3 inhibited both activities, whereas 4 and 5 were inhibitory only in the transcription activity at concentrations higher than 10/ig/ml. However 6 had no effects on both activities.

AB - We examined the effects of diethylstilbestrol (1), Z,Z-dienestrol (2), E,E-dienestrol (3), indenestrol A (4), indenestrol B (5) and estradiol (6) on the activity of in vitro accurate transcription from adenovirus 2 major late promoter by mammalian ribonucleic acid (RNA) polymerase II using partially purified transcription factors from HeLa cell nuclear extract as well as on RNA polymerase II activity assayed for random incorporation of ribonucleotides. 1, 2, and 3 inhibited both activities, whereas 4 and 5 were inhibitory only in the transcription activity at concentrations higher than 10/ig/ml. However 6 had no effects on both activities.

KW - RNA polymerase II

KW - dienestrol

KW - diethylstilbestrol

KW - estradiol

KW - indenestrol A

KW - indenestrol B

KW - transcription

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Inhibition of Transcription by Mammalian Ribonucleic Acid Polymerase II: Effects of Diethylstilbestrol and Its Analogues

Abstract

Keywords

ASJC Scopus subject areas

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