Inhibitory effects of fullerene C60 derivatives on endothelium-derived relaxation in rabbit thoracic aorta

Mitsutoshi Satoh; Tadahiko Mashino; Tetsuo Nagano; Masaaki Hirobe; Issei Takayanagi; Katsuo Koike

doi:10.1081/FST-100102961

Inhibitory effects of fullerene C₆₀ derivatives on endothelium-derived relaxation in rabbit thoracic aorta

Mitsutoshi Satoh, Tadahiko Mashino, Tetsuo Nagano, Masaaki Hirobe, Issei Takayanagi, Katsuo Koike

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Inhibitory effects of newly synthesized fullerene C₆₀ derivatives 1 (C₆₀-bis(N, N-dimethylpyrrolidinium iodide)), 2 (C₆₀-proline-N-acetic acid) and 3 (C₆₀-ethylenediamine-N, N′-diacetic acid) on acetylcholine-induced relaxation in endothelium-intact rabbit thoracic aorta precontracted by phenylephrine (10^-6 M) were studied. Fullerene C₆₀ derivative 1 (3 × 10^-6 M), 2 (10^-5 M) and 3 (10^-5 M) reduced the maximum amplitude of the acetylcholine-induced relaxation without significantly changing the pD₂ values obtained from the concentration - response curves. In the presence of fullerene C₆₀ derivative 1 (10^-5 M) the acetylcholine-induced relaxation was eliminated and an acetylcholine-induced contraction was observed. These results suggest that fullerene C₆₀ derivative 1 strongly inhibits endothelium (nitric oxide)-dependent acetylcholine-induced relaxation in thoracic aorta of rabbit.

Original language	English
Pages (from-to)	141-151
Number of pages	11
Journal	Fullerene Science and Technology
Volume	9
Issue number	2
DOIs	https://doi.org/10.1081/FST-100102961
Publication status	Published - 2001

Keywords

Endothelium
Fullerene C derivative
Nitric oxide (NO)
Smooth muscle
Thoracic aorta of rabbit

ASJC Scopus subject areas

Chemical Engineering(all)

Access to Document

10.1081/FST-100102961

Cite this

@article{13b1fae84474482bae9cc4f034090176,

title = "Inhibitory effects of fullerene C60 derivatives on endothelium-derived relaxation in rabbit thoracic aorta",

abstract = "Inhibitory effects of newly synthesized fullerene C60 derivatives 1 (C60-bis(N, N-dimethylpyrrolidinium iodide)), 2 (C60-proline-N-acetic acid) and 3 (C60-ethylenediamine-N, N′-diacetic acid) on acetylcholine-induced relaxation in endothelium-intact rabbit thoracic aorta precontracted by phenylephrine (10-6 M) were studied. Fullerene C60 derivative 1 (3 × 10-6 M), 2 (10-5 M) and 3 (10-5 M) reduced the maximum amplitude of the acetylcholine-induced relaxation without significantly changing the pD2 values obtained from the concentration - response curves. In the presence of fullerene C60 derivative 1 (10-5 M) the acetylcholine-induced relaxation was eliminated and an acetylcholine-induced contraction was observed. These results suggest that fullerene C60 derivative 1 strongly inhibits endothelium (nitric oxide)-dependent acetylcholine-induced relaxation in thoracic aorta of rabbit.",

keywords = "Endothelium, Fullerene C derivative, Nitric oxide (NO), Smooth muscle, Thoracic aorta of rabbit",

author = "Mitsutoshi Satoh and Tadahiko Mashino and Tetsuo Nagano and Masaaki Hirobe and Issei Takayanagi and Katsuo Koike",

note = "Funding Information: This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (Nos. 11672191 and 90165697).",

year = "2001",

doi = "10.1081/FST-100102961",

language = "English",

volume = "9",

pages = "141--151",

journal = "Fullerene Science and Technology",

issn = "1064-122X",

publisher = "Taylor and Francis Ltd.",

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TY - JOUR

T1 - Inhibitory effects of fullerene C60 derivatives on endothelium-derived relaxation in rabbit thoracic aorta

AU - Satoh, Mitsutoshi

AU - Mashino, Tadahiko

AU - Nagano, Tetsuo

AU - Hirobe, Masaaki

AU - Takayanagi, Issei

AU - Koike, Katsuo

N1 - Funding Information: This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (Nos. 11672191 and 90165697).

PY - 2001

Y1 - 2001

N2 - Inhibitory effects of newly synthesized fullerene C60 derivatives 1 (C60-bis(N, N-dimethylpyrrolidinium iodide)), 2 (C60-proline-N-acetic acid) and 3 (C60-ethylenediamine-N, N′-diacetic acid) on acetylcholine-induced relaxation in endothelium-intact rabbit thoracic aorta precontracted by phenylephrine (10-6 M) were studied. Fullerene C60 derivative 1 (3 × 10-6 M), 2 (10-5 M) and 3 (10-5 M) reduced the maximum amplitude of the acetylcholine-induced relaxation without significantly changing the pD2 values obtained from the concentration - response curves. In the presence of fullerene C60 derivative 1 (10-5 M) the acetylcholine-induced relaxation was eliminated and an acetylcholine-induced contraction was observed. These results suggest that fullerene C60 derivative 1 strongly inhibits endothelium (nitric oxide)-dependent acetylcholine-induced relaxation in thoracic aorta of rabbit.

AB - Inhibitory effects of newly synthesized fullerene C60 derivatives 1 (C60-bis(N, N-dimethylpyrrolidinium iodide)), 2 (C60-proline-N-acetic acid) and 3 (C60-ethylenediamine-N, N′-diacetic acid) on acetylcholine-induced relaxation in endothelium-intact rabbit thoracic aorta precontracted by phenylephrine (10-6 M) were studied. Fullerene C60 derivative 1 (3 × 10-6 M), 2 (10-5 M) and 3 (10-5 M) reduced the maximum amplitude of the acetylcholine-induced relaxation without significantly changing the pD2 values obtained from the concentration - response curves. In the presence of fullerene C60 derivative 1 (10-5 M) the acetylcholine-induced relaxation was eliminated and an acetylcholine-induced contraction was observed. These results suggest that fullerene C60 derivative 1 strongly inhibits endothelium (nitric oxide)-dependent acetylcholine-induced relaxation in thoracic aorta of rabbit.

KW - Endothelium

KW - Fullerene C derivative

KW - Nitric oxide (NO)

KW - Smooth muscle

KW - Thoracic aorta of rabbit

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