Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome: An efficient selection method

Rie Uotsu-Tomita; Shinya Kaneko; Kenji Tsuge; Mitsuhiro Itaya

doi:10.1271/bbb.69.1036

Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome: An efficient selection method

Rie Uotsu-Tomita, Shinya Kaneko, Kenji Tsuge, Mitsuhiro Itaya

Faculty of Environment and Information Studies

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.

Original language	English
Pages (from-to)	1036-1039
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	69
Issue number	5
DOIs	https://doi.org/10.1271/bbb.69.1036
Publication status	Published - 2005 May

Keywords

Bacillus subtilis genome
Inositol
Phleomycin
Transformation
pBR322

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.69.1036

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abstract = "The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.",

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AU - Tsuge, Kenji

AU - Itaya, Mitsuhiro

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KW - Inositol

KW - Phleomycin

KW - Transformation

KW - pBR322

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Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome: An efficient selection method

Abstract

Keywords

ASJC Scopus subject areas

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