Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells

Ryota Kawahara; Yuki Niwa; Siro Simizu

doi:10.1111/cas.13693

Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells

Ryota Kawahara, Yuki Niwa, Siro Simizu

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Vasculogenic mimicry (VM) formation by cancer cells is known to play a crucial role in tumor progression, but its detailed mechanism is unclear. In the present study, we focused on integrin β1 (ITGB1) and assessed the role of ITGB1 in VM formation. We used in vitro methods to seed cancer cells on Matrigel to evaluate the capability of VM formation. We carried out ITGB1 gene deletion using the CRISPR/Cas9 system, and these ITGB1-knockout cells did not show a VM-like network formation. Further, reintroduction of ITGB1 rescued VM-like network formation in ITGB1-knockout cells. In conclusion, ITGB1 is a critical factor in VM of human cancer cells, and inhibition of ITGB1 may be a novel therapeutic approach for malignant cancer.

Original language	English
Pages (from-to)	2490-2496
Number of pages	7
Journal	Cancer science
Volume	109
Issue number	8
DOIs	https://doi.org/10.1111/cas.13693
Publication status	Published - 2018 Aug

Keywords

CRISPR/Cas9
focal adhesion kinase
integrin β1
metastasis
vasculogenic mimicry

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/cas.13693

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title = "Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells",

abstract = "Vasculogenic mimicry (VM) formation by cancer cells is known to play a crucial role in tumor progression, but its detailed mechanism is unclear. In the present study, we focused on integrin β1 (ITGB1) and assessed the role of ITGB1 in VM formation. We used in vitro methods to seed cancer cells on Matrigel to evaluate the capability of VM formation. We carried out ITGB1 gene deletion using the CRISPR/Cas9 system, and these ITGB1-knockout cells did not show a VM-like network formation. Further, reintroduction of ITGB1 rescued VM-like network formation in ITGB1-knockout cells. In conclusion, ITGB1 is a critical factor in VM of human cancer cells, and inhibition of ITGB1 may be a novel therapeutic approach for malignant cancer.",

keywords = "CRISPR/Cas9, focal adhesion kinase, integrin β1, metastasis, vasculogenic mimicry",

author = "Ryota Kawahara and Yuki Niwa and Siro Simizu",

note = "Funding Information: Funding information Japan Society for the Promotion of Science (Grant/Award Number: {\textquoteleft}Grant-in-Aid for Young Scientists (B) (JP17K15094){\textquoteright} This work was supported by a Grant-in-Aid for Young Scientists (B) under Grant Number JP17K15094 (to YN). Publisher Copyright: {\textcopyright} 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.",

year = "2018",

month = aug,

doi = "10.1111/cas.13693",

language = "English",

volume = "109",

pages = "2490--2496",

journal = "Cancer science",

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T1 - Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells

AU - Kawahara, Ryota

AU - Niwa, Yuki

AU - Simizu, Siro

N1 - Funding Information: Funding information Japan Society for the Promotion of Science (Grant/Award Number: ‘Grant-in-Aid for Young Scientists (B) (JP17K15094)’ This work was supported by a Grant-in-Aid for Young Scientists (B) under Grant Number JP17K15094 (to YN). Publisher Copyright: © 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

PY - 2018/8

Y1 - 2018/8

N2 - Vasculogenic mimicry (VM) formation by cancer cells is known to play a crucial role in tumor progression, but its detailed mechanism is unclear. In the present study, we focused on integrin β1 (ITGB1) and assessed the role of ITGB1 in VM formation. We used in vitro methods to seed cancer cells on Matrigel to evaluate the capability of VM formation. We carried out ITGB1 gene deletion using the CRISPR/Cas9 system, and these ITGB1-knockout cells did not show a VM-like network formation. Further, reintroduction of ITGB1 rescued VM-like network formation in ITGB1-knockout cells. In conclusion, ITGB1 is a critical factor in VM of human cancer cells, and inhibition of ITGB1 may be a novel therapeutic approach for malignant cancer.

AB - Vasculogenic mimicry (VM) formation by cancer cells is known to play a crucial role in tumor progression, but its detailed mechanism is unclear. In the present study, we focused on integrin β1 (ITGB1) and assessed the role of ITGB1 in VM formation. We used in vitro methods to seed cancer cells on Matrigel to evaluate the capability of VM formation. We carried out ITGB1 gene deletion using the CRISPR/Cas9 system, and these ITGB1-knockout cells did not show a VM-like network formation. Further, reintroduction of ITGB1 rescued VM-like network formation in ITGB1-knockout cells. In conclusion, ITGB1 is a critical factor in VM of human cancer cells, and inhibition of ITGB1 may be a novel therapeutic approach for malignant cancer.

KW - CRISPR/Cas9

KW - focal adhesion kinase

KW - integrin β1

KW - metastasis

KW - vasculogenic mimicry

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Integrin β1 is an essential factor in vasculogenic mimicry of human cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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