TY - JOUR
T1 - Interleukin-1 receptor immunoreactivity in sympathetic vascular and non-vascular neurons in guinea-pig coeliac ganglion
AU - Morris, Judy L.
AU - Jobling, Phillip
AU - Shimizu, Toshihiko
AU - Gibbins, Ian L.
N1 - Funding Information:
Supported by a Grant in Aid from the National Heart Foundation of Australia (G01A0261). JLM is a Principal Research Fellow of the National Health and Medical Research Council of Australia. We are grateful to Nina Nguyen and Pat Vilimas for excellent technical assistance, and to Drs J. Brown, W. Blessing, J. Oliver and R. Murphy for generous provision of antisera.
PY - 2002/11/15
Y1 - 2002/11/15
N2 - Immunoreactivity (IR) for the interleukin-1 receptor type I (IL1RI) was examined in sympathetic neurons in guinea-pig coeliac ganglion using multiple-labelling immunofluorescence. IL1RI-IR was present in 8% of sympathetic neurons in untreated preparations. The proportion of neurons with IL1RI-IR increased significantly after incubation in interleukin-6 (200 ng/ml) for 2-4 h (16-26% neurons), or after incubation for 4 h without cytokine (16%), with interleukin-1β (IL1β, 200 ng/ml; 18%) or tumour necrosis factor-α (200 ng/ml; 16%). This increase occurred predominantly in neuropeptide Y-IR, vasoconstrictor neurons. IL1RI-IR also was present in varicose axons, some of which projected from the gut, and in vascular smooth muscle cells and endothelium. These potential binding sites for the proinflammatory cytokine, IL1β, on vasoconstrictor neurons and blood vessels may modulate sympathetic regulation of intestinal blood flow in inflammatory conditions.
AB - Immunoreactivity (IR) for the interleukin-1 receptor type I (IL1RI) was examined in sympathetic neurons in guinea-pig coeliac ganglion using multiple-labelling immunofluorescence. IL1RI-IR was present in 8% of sympathetic neurons in untreated preparations. The proportion of neurons with IL1RI-IR increased significantly after incubation in interleukin-6 (200 ng/ml) for 2-4 h (16-26% neurons), or after incubation for 4 h without cytokine (16%), with interleukin-1β (IL1β, 200 ng/ml; 18%) or tumour necrosis factor-α (200 ng/ml; 16%). This increase occurred predominantly in neuropeptide Y-IR, vasoconstrictor neurons. IL1RI-IR also was present in varicose axons, some of which projected from the gut, and in vascular smooth muscle cells and endothelium. These potential binding sites for the proinflammatory cytokine, IL1β, on vasoconstrictor neurons and blood vessels may modulate sympathetic regulation of intestinal blood flow in inflammatory conditions.
KW - Interleukin-1 receptor type I
KW - Interleukin-1β
KW - Interleukin-6
KW - Mesenteric circulation
KW - Proinflammatory cytokines
KW - Sympathetic neurons
KW - Tumour necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=0037110964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037110964&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(02)00959-X
DO - 10.1016/S0304-3940(02)00959-X
M3 - Article
C2 - 12401559
AN - SCOPUS:0037110964
SN - 0304-3940
VL - 333
SP - 54
EP - 58
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -