Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

Hirokazu Komatsu; Yutaka Shindo; Shigehiro A. Kawashima; Kenzo Yamatsugu; Kotaro Oka; Motomu Kanai

doi:10.1039/c3cc40616d

Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

Hirokazu Komatsu, Yutaka Shindo, Shigehiro A. Kawashima, Kenzo Yamatsugu, Kotaro Oka, Motomu Kanai

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The application of a new rhodamine-based fluorescent probe, RH–NH₂3and an acyl transfer promoter, PBu₃, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH–NH₂3with activated acetyl-CoA by the artificial reaction promoter in living cells.

Original language	English
Pages (from-to)	2876-2878
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	28
DOIs	https://doi.org/10.1039/c3cc40616d
Publication status	Published - 2013 Mar 12

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection",

abstract = "The application of a new rhodamine-based fluorescent probe, RH–NH23and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH–NH23with activated acetyl-CoA by the artificial reaction promoter in living cells.",

author = "Hirokazu Komatsu and Yutaka Shindo and Kawashima, {Shigehiro A.} and Kenzo Yamatsugu and Kotaro Oka and Motomu Kanai",

year = "2013",

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T1 - Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

AU - Komatsu, Hirokazu

AU - Shindo, Yutaka

AU - Kawashima, Shigehiro A.

AU - Yamatsugu, Kenzo

AU - Oka, Kotaro

AU - Kanai, Motomu

PY - 2013/3/12

Y1 - 2013/3/12

N2 - The application of a new rhodamine-based fluorescent probe, RH–NH23and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH–NH23with activated acetyl-CoA by the artificial reaction promoter in living cells.

AB - The application of a new rhodamine-based fluorescent probe, RH–NH23and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH–NH23with activated acetyl-CoA by the artificial reaction promoter in living cells.

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U2 - 10.1039/c3cc40616d

DO - 10.1039/c3cc40616d

M3 - Article

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AN - SCOPUS:84875031611

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JO - Chemical Communications

JF - Chemical Communications

IS - 28

ER -

Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

Abstract

ASJC Scopus subject areas

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