Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, Yutaka Kawakami

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalMethods in Molecular Biology
Publication statusPublished - 2014


  • Cryoablation
  • Dendritic cells
  • Immunotherapy
  • KLH

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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