Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura; Masaru Udagawa; Tomonobu Fujita; Toshiharu Sakurai; Tomonori Yaguchi; Yutaka Kawakami

doi:10.1007/978-1-4939-0345-0_13

Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura
, Masaru Udagawa
, Tomonobu Fujita
, Toshiharu Sakurai
, Tomonori Yaguchi
, Yutaka Kawakami

Division of Cellular Signaling

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

Original language	English
Pages (from-to)	145-153
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1139
DOIs	https://doi.org/10.1007/978-1-4939-0345-0_13
Publication status	Published - 2014

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

BCG-CWS
Cryoablation
Dendritic cells
Immunotherapy
KLH

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-0345-0_13

Cite this

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abstract = "Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.",

keywords = "BCG-CWS, Cryoablation, Dendritic cells, Immunotherapy, KLH",

author = "Naoshi Kawamura and Masaru Udagawa and Tomonobu Fujita and Toshiharu Sakurai and Tomonori Yaguchi and Yutaka Kawakami",

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T1 - Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

AU - Kawamura, Naoshi

AU - Udagawa, Masaru

AU - Fujita, Tomonobu

AU - Sakurai, Toshiharu

AU - Yaguchi, Tomonori

AU - Kawakami, Yutaka

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

AB - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

KW - BCG-CWS

KW - Cryoablation

KW - Dendritic cells

KW - Immunotherapy

KW - KLH

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UR - https://www.scopus.com/pages/publications/84909636878#tab=citedBy

U2 - 10.1007/978-1-4939-0345-0_13

DO - 10.1007/978-1-4939-0345-0_13

M3 - Article

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AN - SCOPUS:84909636878

SN - 1064-3745

VL - 1139

SP - 145

EP - 153

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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