TY - JOUR
T1 - Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex
AU - Waki, Michihiko Luca
AU - Onoue, Kenji
AU - Takahashi, Tsukasa
AU - Goto, Kensuke
AU - Saito, Yusuke
AU - Inami, Katsuaki
AU - Makita, Ippei
AU - Angata, Yurika
AU - Suzuki, Tomomi
AU - Yamashita, Mihi
AU - Sato, Narumi
AU - Nakamura, Saki
AU - Yuki, Dai
AU - Sugiura, Yuki
AU - Zaima, Nobuhiro
AU - Goto-Inoue, Naoko
AU - Hayasaka, Takahiro
AU - Shimomura, Yutaka
AU - Setou, Mitsutoshi
PY - 2011/10/24
Y1 - 2011/10/24
N2 - Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.
AB - Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.
UR - http://www.scopus.com/inward/record.url?scp=80055049927&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80055049927&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0026721
DO - 10.1371/journal.pone.0026721
M3 - Article
C2 - 22039541
AN - SCOPUS:80055049927
SN - 1932-6203
VL - 6
JO - PloS one
JF - PloS one
IS - 10
M1 - e26721
ER -