Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts

Kento Mori; Hongkai Sun; Kazuki Miura; Siro Simizu

doi:10.3390/molecules26185685

Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts

Kento Mori, Hongkai Sun, Kazuki Miura, Siro Simizu

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

Original language	English
Article number	5685
Journal	Molecules
Volume	26
Issue number	18
DOIs	https://doi.org/10.3390/molecules26185685
Publication status	Published - 2021 Sept

Keywords

C-mannosylation
DPY19L3
Glycobiology
Myogenic differentiation
Myotube

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules26185685

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title = "Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts",

abstract = "DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.",

keywords = "C-mannosylation, DPY19L3, Glycobiology, Myogenic differentiation, Myotube",

author = "Kento Mori and Hongkai Sun and Kazuki Miura and Siro Simizu",

note = "Funding Information: Funding: This research was funded by the Grant-in-Aid for Scientific Research (C) under Grant Number JP18K06137 and the Mizutani Foundation for Glycoscience. Funding Information: Acknowledgments: This research was funded by Keio University Academic Development Funds. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = sep,

doi = "10.3390/molecules26185685",

language = "English",

volume = "26",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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T1 - Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts

AU - Mori, Kento

AU - Sun, Hongkai

AU - Miura, Kazuki

AU - Simizu, Siro

N1 - Funding Information: Funding: This research was funded by the Grant-in-Aid for Scientific Research (C) under Grant Number JP18K06137 and the Mizutani Foundation for Glycoscience. Funding Information: Acknowledgments: This research was funded by Keio University Academic Development Funds. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/9

Y1 - 2021/9

N2 - DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

AB - DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

KW - C-mannosylation

KW - DPY19L3

KW - Glycobiology

KW - Myogenic differentiation

KW - Myotube

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Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts

Abstract

Keywords

ASJC Scopus subject areas

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