Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

Tomoko Taguchi; Hisami Takenouchi; Jun Matsui; Wei Ran Tang; Mitsuko Itagaki; Yusuke Shiozawa; Kyoko Suzuki; Sachi Sakaguchi; Yohko U. Ktagiri; Takao Takahashi; Hajime Okita; Junichiro Fujimoto; Nobutaka Kiyokawa

doi:10.1016/j.exphem.2006.01.009

Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

Tomoko Taguchi, Hisami Takenouchi, Jun Matsui, Wei Ran Tang, Mitsuko Itagaki, Yusuke Shiozawa, Kyoko Suzuki, Sachi Sakaguchi, Yohko U. Ktagiri, Takao Takahashi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34⁺ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34⁺ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34⁺ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

Original language	English
Pages (from-to)	508-518
Number of pages	11
Journal	Experimental Hematology
Volume	34
Issue number	4
DOIs	https://doi.org/10.1016/j.exphem.2006.01.009
Publication status	Published - 2006 Apr

ASJC Scopus subject areas

Molecular Biology
Hematology
Genetics
Cell Biology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exphem.2006.01.009

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Taguchi, T., Takenouchi, H., Matsui, J., Tang, W. R., Itagaki, M., Shiozawa, Y., Suzuki, K., Sakaguchi, S., Ktagiri, Y. U., Takahashi, T., Okita, H., Fujimoto, J., & Kiyokawa, N. (2006). Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. Experimental Hematology, 34(4), 508-518. https://doi.org/10.1016/j.exphem.2006.01.009

Taguchi, T, Takenouchi, H, Matsui, J, Tang, WR, Itagaki, M, Shiozawa, Y, Suzuki, K, Sakaguchi, S, Ktagiri, YU, Takahashi, T, Okita, H, Fujimoto, J & Kiyokawa, N 2006, 'Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development', Experimental Hematology, vol. 34, no. 4, pp. 508-518. https://doi.org/10.1016/j.exphem.2006.01.009

@article{7d002c423cd8465c91534159e4506724,

title = "Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development",

abstract = "Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.",

author = "Tomoko Taguchi and Hisami Takenouchi and Jun Matsui and Tang, {Wei Ran} and Mitsuko Itagaki and Yusuke Shiozawa and Kyoko Suzuki and Sachi Sakaguchi and Ktagiri, {Yohko U.} and Takao Takahashi and Hajime Okita and Junichiro Fujimoto and Nobutaka Kiyokawa",

note = "Funding Information: We thank S. Yamauchi for her excellent secretarial work. We also thank Dr. A. Manabe, Dr. K. J. Mori, and Dr. Y. Matsuo for gifting murine BM stromal cell line, MS-5, and human leukemia cell line HPB-NULL. This work is supported in part by MEXT. KAKENHI 16017321, JSPS. KAKENHI 17591131, the Budget for Nuclear Research of the Ministry of Education, Culture, Sports, Science and Technology, based on the screening and counseling by the Atomic Energy Commission, grant from the Japan Health Sciences Foundation for Research on Health Sciences Focusing on Drug Innovation, and a Grant for Child Health and Development from the Ministry of Health, Labour and Welfare. T. Taguchi is an Awardee of Research Resident Fellowship from the Foundation for Promotion of Cancer Research (Japan) for the 3rd Term Comprehensive 10-Years-Strategy for Cancer Control.",

year = "2006",

month = apr,

doi = "10.1016/j.exphem.2006.01.009",

language = "English",

volume = "34",

pages = "508--518",

journal = "Experimental Hematology",

issn = "0301-472X",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

AU - Taguchi, Tomoko

AU - Takenouchi, Hisami

AU - Matsui, Jun

AU - Tang, Wei Ran

AU - Itagaki, Mitsuko

AU - Shiozawa, Yusuke

AU - Suzuki, Kyoko

AU - Sakaguchi, Sachi

AU - Ktagiri, Yohko U.

AU - Takahashi, Takao

AU - Okita, Hajime

AU - Fujimoto, Junichiro

AU - Kiyokawa, Nobutaka

N1 - Funding Information: We thank S. Yamauchi for her excellent secretarial work. We also thank Dr. A. Manabe, Dr. K. J. Mori, and Dr. Y. Matsuo for gifting murine BM stromal cell line, MS-5, and human leukemia cell line HPB-NULL. This work is supported in part by MEXT. KAKENHI 16017321, JSPS. KAKENHI 17591131, the Budget for Nuclear Research of the Ministry of Education, Culture, Sports, Science and Technology, based on the screening and counseling by the Atomic Energy Commission, grant from the Japan Health Sciences Foundation for Research on Health Sciences Focusing on Drug Innovation, and a Grant for Child Health and Development from the Ministry of Health, Labour and Welfare. T. Taguchi is an Awardee of Research Resident Fellowship from the Foundation for Promotion of Cancer Research (Japan) for the 3rd Term Comprehensive 10-Years-Strategy for Cancer Control.

PY - 2006/4

Y1 - 2006/4

N2 - Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

AB - Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

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Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

Abstract

ASJC Scopus subject areas

UN SDGs

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