Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings

Toyoaki Anai; Naoko Kono; Seiji Kosemura; Shosuke Yamahura; Koji Hasegawa

doi:10.3109/10425179809008472

Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings

Toyoaki Anai, Naoko Kono, Seiji Kosemura, Shosuke Yamahura, Koji Hasegawa

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the J'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promotem were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.

Original language	English
Pages (from-to)	329-333
Number of pages	5
Journal	Mitochondrial DNA
Volume	9
Issue number	5-6
DOIs	https://doi.org/10.3109/10425179809008472
Publication status	Published - 1998
Externally published	Yes

Keywords

Raphanus sativus
SAW auxin-inducible gene
promoter region

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.3109/10425179809008472

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title = "Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings",

abstract = "A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the J'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promotem were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.",

keywords = "Raphanus sativus, SAW auxin-inducible gene, promoter region",

author = "Toyoaki Anai and Naoko Kono and Seiji Kosemura and Shosuke Yamahura and Koji Hasegawa",

note = "Funding Information: The authors thank Drs. H. Uchida, K. Yoko-tani-Tomita and K. Yamada for helpful discus- sion. This work was supported in part by a Grant-in Aid for Scientific Research on priority Areas (No. 06240103) from the Ministry of Education, Science and Culture Japan to K. H.",

year = "1998",

doi = "10.3109/10425179809008472",

language = "English",

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AU - Anai, Toyoaki

AU - Kono, Naoko

AU - Kosemura, Seiji

AU - Yamahura, Shosuke

AU - Hasegawa, Koji

N1 - Funding Information: The authors thank Drs. H. Uchida, K. Yoko-tani-Tomita and K. Yamada for helpful discus- sion. This work was supported in part by a Grant-in Aid for Scientific Research on priority Areas (No. 06240103) from the Ministry of Education, Science and Culture Japan to K. H.

PY - 1998

Y1 - 1998

N2 - A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the J'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promotem were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.

AB - A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the J'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promotem were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.

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Isolation and characterization of an auxin-inducible SAUR gene from radish seedlings

Abstract

Keywords

ASJC Scopus subject areas

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