TY - JOUR
T1 - Isolation and Characterization of the gro ES and groEL genes of Bacillus subtilis Marburg
AU - Tozawa, Yuzuru
AU - Yoshikawa, Hirofumi
AU - Kawamura, Fujio
AU - Takahashi, Hideo
AU - Itaya, Mitsuhiro
N1 - Funding Information:
Acknowledgments. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, and Science of Japan and "Biodesign Research Program" from RIKEN.
PY - 1992
Y1 - 1992
N2 - The complete set of gro ES and groEL gene homologues from Bacillus subtilis Marburg 168 was identified, cloned, and characterized. The nucleotide sequence indicated the presence of two open reading frames corresponding to the gro ES and groEL genes. The presumptive gro ES and GroEL proteins were calculated to be Polypeptides of 10,175 and 57,175 Da, respectively, and showed extensive sequence similarities with the known gro ES and GroEL proteins of Escherichia coli and Mycobacterium tuberculosis. A heat-inducible transcript initiated upstream of the groES coding region was identified by primer-extension analysis of in vivo transcripts, indicating that the two genes consist of an Operon. At least six heat-shock inducible proteins were identified in the cell extract of heat treated B. subtilis. Two proteins of 10 and 60 kDa overproduced in B. subtilis cells carrying a multi-copy groES and groEL plasmid were demonstrated to correspond to two out of the six heat-shock inducible proteins. The groES and groEL genes of B. subtilis were physically mapped on the 60° region of a 360° map and genetically mapped at the position of 40% linkage with the purB locus using PBS1 transduction of the groEL genes tagged with a chloramphenicol resistance (chlr) marker.
AB - The complete set of gro ES and groEL gene homologues from Bacillus subtilis Marburg 168 was identified, cloned, and characterized. The nucleotide sequence indicated the presence of two open reading frames corresponding to the gro ES and groEL genes. The presumptive gro ES and GroEL proteins were calculated to be Polypeptides of 10,175 and 57,175 Da, respectively, and showed extensive sequence similarities with the known gro ES and GroEL proteins of Escherichia coli and Mycobacterium tuberculosis. A heat-inducible transcript initiated upstream of the groES coding region was identified by primer-extension analysis of in vivo transcripts, indicating that the two genes consist of an Operon. At least six heat-shock inducible proteins were identified in the cell extract of heat treated B. subtilis. Two proteins of 10 and 60 kDa overproduced in B. subtilis cells carrying a multi-copy groES and groEL plasmid were demonstrated to correspond to two out of the six heat-shock inducible proteins. The groES and groEL genes of B. subtilis were physically mapped on the 60° region of a 360° map and genetically mapped at the position of 40% linkage with the purB locus using PBS1 transduction of the groEL genes tagged with a chloramphenicol resistance (chlr) marker.
UR - http://www.scopus.com/inward/record.url?scp=0027015407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027015407&partnerID=8YFLogxK
U2 - 10.1271/bbb.56.1995
DO - 10.1271/bbb.56.1995
M3 - Article
C2 - 1369494
AN - SCOPUS:0027015407
SN - 0916-8451
VL - 56
SP - 1995
EP - 2002
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 12
ER -
