Isolation and identification of an allelopathic substance from duckweed (Lemna minor L.)

T. T.N. Bich, Osamu Ohno, K. Suenaga, H. Kato-Noguchi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


An aqueous methanol extract of duckweed (Lemna minor L.) inhibited the growth of cress (Lepidium sativum L.). The extract was partitioned by silica gel, Diaion HP20, Sephadex LH-20 and Diaion HP20SS columns, C18 Sep-Pak cartridges and reverse phase HPLC to isolate phytotoxic substances. The (3R)-(-)-3-hydroxy-β- ionone was identified as the active ingredient in the extract by its spectral data. (3R)-(-)-3-hydroxy-β-ionone inhibited growth of cress and Italian ryegrass seedlings at ≥0.1 and ≥5 μM, respectively. (3R)-(-)-3-Hydroxy-β-ionone inhibited 50% of cress roots and shoots growth with 0.1 μM and 50% of Italian ryegrass root and shoot growth at 2.4 and 3.4 μM, respectively. The presence of (3R)-(-)-3-hydroxy-β-ionone in duckweed and its growth inhibitory activity suggested that it may contribute to the allelopathic potential of duckweed.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalAllelopathy Journal
Issue number2
Publication statusPublished - 2013 Dec 1


  • 3-hydroxy-β-ionone
  • Allelopathy
  • Aquatic plant
  • Duckweed
  • Eco-agriculture
  • Growth inhibitor
  • Ionone
  • Italian ryegrass
  • Natural resource
  • Weed management

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


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