Isolation and in vitro expansion of human colonic stem cells

Peter Jung, Toshiro Sato, Anna Merlos-Suárez, Francisco M. Barriga, Mar Iglesias, David Rossell, Herbert Auer, Mercedes Gallardo, Maria A. Blasco, Elena Sancho, Hans Clevers, Eduard Batlle

Research output: Contribution to journalArticlepeer-review

525 Citations (Scopus)


Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.

Original languageEnglish
Pages (from-to)1225-1227
Number of pages3
JournalNature medicine
Issue number10
Publication statusPublished - 2011 Oct

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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