TY - JOUR
T1 - Isolation and sequence determination of rat cardiac natriuretic peptide
AU - Kambayashi, Yoshikazu
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Hosoda, Kiminori
AU - Saito, Yoshihiko
AU - Yamada, Takayuki
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Shirakami, Gotaro
AU - Suga, Shin ichi
AU - Ogawa, Yoshihiro
AU - Jougasaki, Michihisa
AU - Minamino, Naoto
AU - Kangawa, Kenji
AU - Matsuo, Hisayuki
AU - Inouye, Ken
AU - Imura, Hiroo
N1 - Funding Information:
We thank Ms. H. Fumon, Ms. A. Furu, Ms. Y. Yoshida and Ms. N. Sako for the excellent secretarial and technical assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1989, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-1 and 63C-2) from the Japanese Ministry of Health and Welfare.
PY - 1989/8/30
Y1 - 1989/8/30
N2 - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
AB - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.
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U2 - 10.1016/0006-291X(89)92126-8
DO - 10.1016/0006-291X(89)92126-8
M3 - Article
C2 - 2528349
AN - SCOPUS:0024462264
SN - 0006-291X
VL - 163
SP - 233
EP - 240
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -