Isolation and sequence determination of rat cardiac natriuretic peptide

Yoshikazu Kambayashi; Kazuwa Nakao; Hiroshi Itoh; Kiminori Hosoda; Yoshihiko Saito; Takayuki Yamada; Masashi Mukoyama; Hiroshi Arai; Gotaro Shirakami; Shin ichi Suga; Yoshihiro Ogawa; Michihisa Jougasaki; Naoto Minamino; Kenji Kangawa; Hisayuki Matsuo; Ken Inouye; Hiroo Imura

doi:10.1016/0006-291X(89)92126-8

Isolation and sequence determination of rat cardiac natriuretic peptide

Yoshikazu Kambayashi, Kazuwa Nakao, Hiroshi Itoh, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Yoshihiro Ogawa, Michihisa Jougasaki, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Ken Inouye, Hiroo Imura

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Abstract

We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.

Original language	English
Pages (from-to)	233-240
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	163
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)92126-8
Publication status	Published - 1989 Aug 30
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kambayashi, Y., Nakao, K., Itoh, H., Hosoda, K., Saito, Y., Yamada, T., Mukoyama, M., Arai, H., Shirakami, G., Suga, S. I., Ogawa, Y., Jougasaki, M., Minamino, N., Kangawa, K., Matsuo, H., Inouye, K., & Imura, H. (1989). Isolation and sequence determination of rat cardiac natriuretic peptide. Biochemical and Biophysical Research Communications, 163(1), 233-240. https://doi.org/10.1016/0006-291X(89)92126-8

Kambayashi, Y, Nakao, K, Itoh, H, Hosoda, K, Saito, Y, Yamada, T, Mukoyama, M, Arai, H, Shirakami, G, Suga, SI, Ogawa, Y, Jougasaki, M, Minamino, N, Kangawa, K, Matsuo, H, Inouye, K & Imura, H 1989, 'Isolation and sequence determination of rat cardiac natriuretic peptide', Biochemical and Biophysical Research Communications, vol. 163, no. 1, pp. 233-240. https://doi.org/10.1016/0006-291X(89)92126-8

@article{364b78a6b1b54a0a897913f65d53ec61,

title = "Isolation and sequence determination of rat cardiac natriuretic peptide",

abstract = "We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.",

author = "Yoshikazu Kambayashi and Kazuwa Nakao and Hiroshi Itoh and Kiminori Hosoda and Yoshihiko Saito and Takayuki Yamada and Masashi Mukoyama and Hiroshi Arai and Gotaro Shirakami and Suga, {Shin ichi} and Yoshihiro Ogawa and Michihisa Jougasaki and Naoto Minamino and Kenji Kangawa and Hisayuki Matsuo and Ken Inouye and Hiroo Imura",

note = "Funding Information: We thank Ms. H. Fumon, Ms. A. Furu, Ms. Y. Yoshida and Ms. N. Sako for the excellent secretarial and technical assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare {"}Disorders of Adrenal Hormone{"} Research Committee, Japan, 1989, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-1 and 63C-2) from the Japanese Ministry of Health and Welfare.",

year = "1989",

month = aug,

day = "30",

doi = "10.1016/0006-291X(89)92126-8",

language = "English",

volume = "163",

pages = "233--240",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Isolation and sequence determination of rat cardiac natriuretic peptide

AU - Kambayashi, Yoshikazu

AU - Nakao, Kazuwa

AU - Itoh, Hiroshi

AU - Hosoda, Kiminori

AU - Saito, Yoshihiko

AU - Yamada, Takayuki

AU - Mukoyama, Masashi

AU - Arai, Hiroshi

AU - Shirakami, Gotaro

AU - Suga, Shin ichi

AU - Ogawa, Yoshihiro

AU - Jougasaki, Michihisa

AU - Minamino, Naoto

AU - Kangawa, Kenji

AU - Matsuo, Hisayuki

AU - Inouye, Ken

AU - Imura, Hiroo

N1 - Funding Information: We thank Ms. H. Fumon, Ms. A. Furu, Ms. Y. Yoshida and Ms. N. Sako for the excellent secretarial and technical assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1989, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-1 and 63C-2) from the Japanese Ministry of Health and Welfare.

PY - 1989/8/30

Y1 - 1989/8/30

N2 - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.

AB - We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.

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U2 - 10.1016/0006-291X(89)92126-8

DO - 10.1016/0006-291X(89)92126-8

M3 - Article

C2 - 2528349

AN - SCOPUS:0024462264

SN - 0006-291X

VL - 163

SP - 233

EP - 240

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Isolation and sequence determination of rat cardiac natriuretic peptide

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