Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

Masaya Imoto; Kazuo Umezawa; Yoshikazu Takahashi; Hiroshi Naganawa; Hikaru Nakamura; Yumi Koizumi; Yumi Sasaki; Masa Hamada; Tsutomu Sawa; Tomio Takeuchi; Yoichi Iitaka

doi:10.1021/np50070a008

Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

Masaya Imoto, Kazuo Umezawa, Yoshikazu Takahashi, Hiroshi Naganawa, Hikaru Nakamura, Yumi Koizumi, Yumi Sasaki, Masa Hamada, Tsutomu Sawa, Tomio Takeuchi, Yoichi Iitaka

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC₅₀ of 0.5 μg/ml in cultured A431 cells.

Original language	English
Pages (from-to)	825-829
Number of pages	5
Journal	Journal of Natural Products
Volume	53
Issue number	4
DOIs	https://doi.org/10.1021/np50070a008
Publication status	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

Access to Document

10.1021/np50070a008

Cite this

@article{e9d07225667e46a6ba708610d2e45fa7,

title = "Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover",

abstract = "In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC50 of 0.5 μg/ml in cultured A431 cells.",

author = "Masaya Imoto and Kazuo Umezawa and Yoshikazu Takahashi and Hiroshi Naganawa and Hikaru Nakamura and Yumi Koizumi and Yumi Sasaki and Masa Hamada and Tsutomu Sawa and Tomio Takeuchi and Yoichi Iitaka",

year = "1990",

doi = "10.1021/np50070a008",

language = "English",

volume = "53",

pages = "825--829",

journal = "Journal of Natural Products",

issn = "0163-3864",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

AU - Imoto, Masaya

AU - Umezawa, Kazuo

AU - Takahashi, Yoshikazu

AU - Naganawa, Hiroshi

AU - Nakamura, Hikaru

AU - Koizumi, Yumi

AU - Sasaki, Yumi

AU - Hamada, Masa

AU - Sawa, Tsutomu

AU - Takeuchi, Tomio

AU - Iitaka, Yoichi

PY - 1990

Y1 - 1990

N2 - In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC50 of 0.5 μg/ml in cultured A431 cells.

AB - In the course of screening for phosphatidylinosicol turnover inhibitors, we found that a culture broth of a Streptomyces strain strongly inhibited the incorporation of inositol into phosphatidylinositol and its phosphates. The active principle was isolated from the broth through EtOAc extraction, cc, centrifugation partition chromatography, and crystallization. Spectroscopic and crystallographic analysis revealed that it has a novel polyether structure, and we named it inostamycin. Inostamycin inhibited phosphatidylinositol turnover with an IC50 of 0.5 μg/ml in cultured A431 cells.

UR - http://www.scopus.com/inward/record.url?scp=0024998009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024998009&partnerID=8YFLogxK

U2 - 10.1021/np50070a008

DO - 10.1021/np50070a008

M3 - Article

AN - SCOPUS:0024998009

SN - 0163-3864

VL - 53

SP - 825

EP - 829

JO - Journal of Natural Products

JF - Journal of Natural Products

IS - 4

ER -

Isolation and structure determination of inostamycin, a novel inhibitor of phosphatidylinositol turnover

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this