TY - JOUR
T1 - Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia
AU - Shi, Tala
AU - Nishiyama, Keita
AU - Nakamata, Koichi
AU - Aryantini, Ni Putu Desy
AU - Mikumo, Dai
AU - Oda, Yuji
AU - Yamamoto, Yuji
AU - Mukai, Takao
AU - Sujaya, I. Nengah
AU - Urashima, Tadasu
AU - Fukuda, Kenji
N1 - Funding Information:
This study was supported financially by the Global Center of Excellence Frontier Program for Animal Global Health and Hygiene of the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2012
Y1 - 2012
N2 - To explore potential probiotics in the traditional foods of Indonesia, fermented mare milk produced in Sumbawa Island was investigated in this study. Gram stain, catalase activity, gas production, cell morphology, carbohydrate utilization pattern, and 16S rDNA sequencing were performed to identify isolated lactic acid bacteria. To assess their probiotic ability, tolerance of low pH, bile salts, artificial gastrointestinal fluids, and adhesion properties to extracellular matrices, were examined. In total 27 strains, 25 Lactobacillus rhamnosus and two Lactobacillus fermentum, were obtained. Among the isolated lactobacilli, three Lb. rhamnosus strains, FSMM15, FSMM22, and FSMM26, were selected as candidates for probiotics, using Lb. rhamnosus GG as index. In vitro binding assay of the three strains against several extracellular matrix proteins revealed that FSMM15 and FSMM26 gave greater binding ratios of mucin/bovine serum albumin (BSA) and significantly higher adhesive abilities to fibronectin than Lb. rhamnosus GG. FSMM22 showed significantly higher adhesion to laminin than Lb. rhamnosus GG.
AB - To explore potential probiotics in the traditional foods of Indonesia, fermented mare milk produced in Sumbawa Island was investigated in this study. Gram stain, catalase activity, gas production, cell morphology, carbohydrate utilization pattern, and 16S rDNA sequencing were performed to identify isolated lactic acid bacteria. To assess their probiotic ability, tolerance of low pH, bile salts, artificial gastrointestinal fluids, and adhesion properties to extracellular matrices, were examined. In total 27 strains, 25 Lactobacillus rhamnosus and two Lactobacillus fermentum, were obtained. Among the isolated lactobacilli, three Lb. rhamnosus strains, FSMM15, FSMM22, and FSMM26, were selected as candidates for probiotics, using Lb. rhamnosus GG as index. In vitro binding assay of the three strains against several extracellular matrix proteins revealed that FSMM15 and FSMM26 gave greater binding ratios of mucin/bovine serum albumin (BSA) and significantly higher adhesive abilities to fibronectin than Lb. rhamnosus GG. FSMM22 showed significantly higher adhesion to laminin than Lb. rhamnosus GG.
KW - Lactobacillus rhamnosus
KW - Laminin adhesive ability
KW - Probiotic properties
KW - Sumbawa mare
KW - Traditional fermented milk
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U2 - 10.1271/bbb.120385
DO - 10.1271/bbb.120385
M3 - Article
C2 - 23047104
AN - SCOPUS:84868572790
SN - 0916-8451
VL - 76
SP - 1897
EP - 1903
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 10
ER -