TY - JOUR
T1 - Kinetic-Studies on Cyclophellitol Analogs - Mechanism-Based Inactivators
AU - Tai, V. W.F.
AU - Fung, P. H.
AU - Wong, Y. S.
AU - Shing, T. K.M.
PY - 1995/8/1
Y1 - 1995/8/1
N2 - The (1R,6S)- and (1R,2S,6S)-diastereoisomers of cyclophellitol were found to be effective irreversible inactivators of α-D-glucosidase and α-D-mannosidase, respectively. The (1R,6S)-diastereoisomer inactivates brewers yeast α-D-glucosidase according to pseudo-first order kinetics with inactivation constants of Ki = 26.9 μM, ki = 0.401 min-1 while the (1R,2S,6S)-diastereoisomer inactivates jack beans α-D-mannosidase in a similar manner with Ki = 120 μM, ki = 2.85 min-1. The irreversibility of these compounds was evidenced by the lack of reactivation upon dialysis of the inactivated enzyme.
AB - The (1R,6S)- and (1R,2S,6S)-diastereoisomers of cyclophellitol were found to be effective irreversible inactivators of α-D-glucosidase and α-D-mannosidase, respectively. The (1R,6S)-diastereoisomer inactivates brewers yeast α-D-glucosidase according to pseudo-first order kinetics with inactivation constants of Ki = 26.9 μM, ki = 0.401 min-1 while the (1R,2S,6S)-diastereoisomer inactivates jack beans α-D-mannosidase in a similar manner with Ki = 120 μM, ki = 2.85 min-1. The irreversibility of these compounds was evidenced by the lack of reactivation upon dialysis of the inactivated enzyme.
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U2 - 10.1006/bbrc.1995.2113
DO - 10.1006/bbrc.1995.2113
M3 - Article
C2 - 7639733
AN - SCOPUS:0029097682
SN - 0006-291X
VL - 213
SP - 175
EP - 180
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -