KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging

Masayoshi Nakasako; Yuki Takayama; Tomotaka Oroguchi; Yuki Sekiguchi; Amane Kobayashi; Keiya Shirahama; Masaki Yamamoto; Takaaki Hikima; Koji Yonekura; Saori Maki-Yonekura; Yoshiki Kohmura; Yuichi Inubushi; Yukio Takahashi; Akihiro Suzuki; Sachihiro Matsunaga; Yayoi Inui; Kensuke Tono; Takashi Kameshima; Yasumasa Joti; Takahiko Hoshi

doi:10.1063/1.4822123

KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging

Masayoshi Nakasako, Yuki Takayama, Tomotaka Oroguchi, Yuki Sekiguchi, Amane Kobayashi, Keiya Shirahama, Masaki Yamamoto, Takaaki Hikima, Koji Yonekura, Saori Maki-Yonekura, Yoshiki Kohmura, Yuichi Inubushi, Yukio Takahashi, Akihiro Suzuki, Sachihiro Matsunaga, Yayoi Inui, Kensuke Tono, Takashi Kameshima, Yasumasa Joti, Takahiko Hoshi

Department of Physics

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Abstract

We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.

Original language	English
Article number	093705
Journal	Review of Scientific Instruments
Volume	84
Issue number	9
DOIs	https://doi.org/10.1063/1.4822123
Publication status	Published - 2013 Sept

ASJC Scopus subject areas

Instrumentation

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10.1063/1.4822123

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Nakasako, M., Takayama, Y., Oroguchi, T., Sekiguchi, Y., Kobayashi, A., Shirahama, K., Yamamoto, M., Hikima, T., Yonekura, K., Maki-Yonekura, S., Kohmura, Y., Inubushi, Y., Takahashi, Y., Suzuki, A., Matsunaga, S., Inui, Y., Tono, K., Kameshima, T., Joti, Y., & Hoshi, T. (2013). KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging. Review of Scientific Instruments, 84(9), Article 093705. https://doi.org/10.1063/1.4822123

Nakasako, M, Takayama, Y, Oroguchi, T, Sekiguchi, Y, Kobayashi, A, Shirahama, K, Yamamoto, M, Hikima, T, Yonekura, K, Maki-Yonekura, S, Kohmura, Y, Inubushi, Y, Takahashi, Y, Suzuki, A, Matsunaga, S, Inui, Y, Tono, K, Kameshima, T, Joti, Y & Hoshi, T 2013, 'KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging', Review of Scientific Instruments, vol. 84, no. 9, 093705. https://doi.org/10.1063/1.4822123

@article{5f491666da1247a1a4656cedb8c2faae,

title = "KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging",

abstract = "We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.",

author = "Masayoshi Nakasako and Yuki Takayama and Tomotaka Oroguchi and Yuki Sekiguchi and Amane Kobayashi and Keiya Shirahama and Masaki Yamamoto and Takaaki Hikima and Koji Yonekura and Saori Maki-Yonekura and Yoshiki Kohmura and Yuichi Inubushi and Yukio Takahashi and Akihiro Suzuki and Sachihiro Matsunaga and Yayoi Inui and Kensuke Tono and Takashi Kameshima and Yasumasa Joti and Takahiko Hoshi",

note = "Funding Information: M.N. is grateful for Professor Yoshinori Fujiyoshi of Nagoya University and Mr. Masaaki Urabe of JEOL for their kind introduction on devices used in their cryo EM. M.N. and M.Y. thank to the late Mr. Asao Suzuki and Mr. Koji Nishi of Niki-glass company for their kind advice to introduce the storage dewar, to Mr. Takatoshi Takeuchi of Kohzu Precision Co. Ltd. for his help in the design of the KOTOBUKI-1 apparatus, and to Mr. Yasufumi Torizuka of Rigaku-Aihara Co. Ltd. for the construction of the slit system. The authors thank to Professor Yoshinori Nishino of RIKEN (currently at Hokkaido University) for his kind help in the CXDI experiment at SPring-8, and also to Mr. Tetsukon Kin and the members of Engineering Support Team of SACLA for their help in the alignment of the optics and our apparatus. The construction and development of the KOTOBUKI-1 apparatus was supported by the grant for XFEL key technology to M.N. and M.Y. and by X-ray Free Electron Laser Priority Strategy Program from the MEXT to M.N., M.Y., Y.T., T.K., and S.M. In addition, sample preparation and software development are supported the grants from the JSPS to M.N. (Grant Nos. 1920402, 22018027, and 2465414), Y.T. (Grant Nos. 21686060 and 25709507), and S.M. (Grant No. 23370029), and from the MEXT to M.N. (Grant Nos. 15076210, 20050030, and 22244054), T.O. (Grant No. 24113723), Y.T. (Grant No. 23102504) and S.M. (Grant Nos. 23120518, 25120726, and 25114514). Experiments to develop the KOTOBUKI-1 apparatus were performed as the experimental proposals for RIKEN beamline of SPring-8 (Grant Nos. 20090097, 20100035, and 20110006) and for SACLA (Grant Nos. 2012A8001, 2012A8005, 2012A8006, 2012A8010, 2012A8022, 2012A8027, 2012B8037, and 2013A8043).",

year = "2013",

month = sep,

doi = "10.1063/1.4822123",

language = "English",

volume = "84",

journal = "Review of Scientific Instruments",

issn = "0034-6748",

publisher = "American Institute of Physics Publising LLC",

number = "9",

}

TY - JOUR

T1 - KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging

AU - Nakasako, Masayoshi

AU - Takayama, Yuki

AU - Oroguchi, Tomotaka

AU - Sekiguchi, Yuki

AU - Kobayashi, Amane

AU - Shirahama, Keiya

AU - Yamamoto, Masaki

AU - Hikima, Takaaki

AU - Yonekura, Koji

AU - Maki-Yonekura, Saori

AU - Kohmura, Yoshiki

AU - Inubushi, Yuichi

AU - Takahashi, Yukio

AU - Suzuki, Akihiro

AU - Matsunaga, Sachihiro

AU - Inui, Yayoi

AU - Tono, Kensuke

AU - Kameshima, Takashi

AU - Joti, Yasumasa

AU - Hoshi, Takahiko

N1 - Funding Information: M.N. is grateful for Professor Yoshinori Fujiyoshi of Nagoya University and Mr. Masaaki Urabe of JEOL for their kind introduction on devices used in their cryo EM. M.N. and M.Y. thank to the late Mr. Asao Suzuki and Mr. Koji Nishi of Niki-glass company for their kind advice to introduce the storage dewar, to Mr. Takatoshi Takeuchi of Kohzu Precision Co. Ltd. for his help in the design of the KOTOBUKI-1 apparatus, and to Mr. Yasufumi Torizuka of Rigaku-Aihara Co. Ltd. for the construction of the slit system. The authors thank to Professor Yoshinori Nishino of RIKEN (currently at Hokkaido University) for his kind help in the CXDI experiment at SPring-8, and also to Mr. Tetsukon Kin and the members of Engineering Support Team of SACLA for their help in the alignment of the optics and our apparatus. The construction and development of the KOTOBUKI-1 apparatus was supported by the grant for XFEL key technology to M.N. and M.Y. and by X-ray Free Electron Laser Priority Strategy Program from the MEXT to M.N., M.Y., Y.T., T.K., and S.M. In addition, sample preparation and software development are supported the grants from the JSPS to M.N. (Grant Nos. 1920402, 22018027, and 2465414), Y.T. (Grant Nos. 21686060 and 25709507), and S.M. (Grant No. 23370029), and from the MEXT to M.N. (Grant Nos. 15076210, 20050030, and 22244054), T.O. (Grant No. 24113723), Y.T. (Grant No. 23102504) and S.M. (Grant Nos. 23120518, 25120726, and 25114514). Experiments to develop the KOTOBUKI-1 apparatus were performed as the experimental proposals for RIKEN beamline of SPring-8 (Grant Nos. 20090097, 20100035, and 20110006) and for SACLA (Grant Nos. 2012A8001, 2012A8005, 2012A8006, 2012A8010, 2012A8022, 2012A8027, 2012B8037, and 2013A8043).

PY - 2013/9

Y1 - 2013/9

N2 - We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.

AB - We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.

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U2 - 10.1063/1.4822123

DO - 10.1063/1.4822123

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AN - SCOPUS:84885152332

SN - 0034-6748

VL - 84

JO - Review of Scientific Instruments

JF - Review of Scientific Instruments

IS - 9

M1 - 093705

ER -

KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging

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