Abstract
The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.
| Translated title of the contribution | Macrophage inflammatory protein-1α (MIP-1α) and leukemia inhibitory factor (LIF) protect the repopulating ability of purified murine hematopoietic stem cells in serum-deprived cultures stimulated with SCF and IL-3 |
|---|---|
| Original language | Italian |
| Pages (from-to) | 553-562 |
| Number of pages | 10 |
| Journal | Annali dell'Istituto Superiore di Sanita |
| Volume | 35 |
| Issue number | 4 |
| Publication status | Published - 1999 Dec 1 |
| Externally published | Yes |
Keywords
- Bone marrow transplantation
- Ex vivo expansion
- Hematopoietic stem cells
- Leukemia inhibitory factor
- Macrophage inflammatory protein-1α
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
