Lack of association between CTLA-4 gene polymorphism and IDDM in Japanese subjects

Tatsuo Yanagawa, Taro Maruyama, Kiyohide Gomi, Matsuo Taniyama, Akira Kasuga, Yukako Ozawa, Masako Terauchi, Hiroshi Hirose, Hiroshi Maruyama, Takao Saruta

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


Susceptibility to insulin-dependent diabetes mellitus (IDDM) is determined by both environmental and genetic factors. The main gene associated with predisposition to IDDM is HLA. Recent studies have described linkage and association of IDDM to the CTLA-4 gene (IDDM12) in Caucasians. CTLA-4 is a candidate gene for T-cell-mediated autoimmune diseases because it is a negative regulator of T-cell proliferation. We investigated the distribution of a CTLA-4 gene polymorphism in 110 Japanese patients with IDDM and 200 control subjects. In 84 patients, we also investigated associations between this CTLA-4 gene polymorphism and GAD65 antibody positivity. An A/G transition at position 49 of exon 1 was analyzed by the polymerase chain reaction-restriction fragment length polymorphism method. GAD65 antibody was detected using a radioligand binding assay. There was no significant difference in the distribution of CTLA-4 alleles in patients and controls and no difference was observed in prevalence of CTLA-4 alleles when GAD65 antibody-positive and -negative individuals in the IDDM groups were compared. The present study did not support an association between the CTLA-4 gene and IDDM in the Japanese population.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
Issue number1
Publication statusPublished - 1999


  • Autoimmunity
  • CTLA-4 gene
  • IDDM

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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