TY - JOUR
T1 - Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling
AU - Shimazaki, Hiroko
AU - Saito, Kozue
AU - Matsuda, Atsushi
AU - Sawakami, Kazumi
AU - Kariya, Minoru
AU - Segawa, Osamu
AU - Miyashita, Yukiko
AU - Ueda, Tetsuya
AU - Koizuka, Michinori
AU - Nakamura, Kazuhiro
AU - Kaji, Hiroyuki
AU - Tajima, Hideji
AU - Kuno, Atsushi
N1 - Funding Information:
We are grateful to Prof. Tatsuro Irimura for offering anti-sialylated MUC1 monoclonal antibody MY.1E12. We thank Ms. Aya Ueki, Ms. Ayaka Ono, Ms. Shiori Kuno, Ms. Takako Toyoda, Ms. Akiko Hirose, and Ms. Masako Sukegawa of the National Institute of Advanced Industrial Science and Technology for technical assistance. This study was supported by projects for “matching promotion type II” in the Revitalization Promotion Program from the Japan Science and Technology Agency (JST) and “utilizing glycans” in the development of innovative drug discovery technologies (Grant No. 16809263) from the Japan Agency for Medical Research and Development (AMED).
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/9/3
Y1 - 2019/9/3
N2 - A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omics-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of "bead arrays in a single tip." The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan-lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.
AB - A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omics-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of "bead arrays in a single tip." The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan-lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.
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U2 - 10.1021/acs.analchem.9b01876
DO - 10.1021/acs.analchem.9b01876
M3 - Article
C2 - 31405276
AN - SCOPUS:85071787731
SN - 0003-2700
VL - 91
SP - 11162
EP - 11169
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 17
ER -