Maternal methamphetamine administration during pregnancy on fetal rat heart development

Hiromasa Inoue, Masato Nakatome, Masaru Terada, Mizuki Mizuno, Reiko Ono, Morio Iino, Yukiko Ino, Yoshiaki Ogura, Hisanaga Kuroki, Ryoji Matoba

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Methamphetamine (MAP) is one of the most abused drugs in Japan. The rate of MAP abuse by young women has recently reached more than 50 percent in adolescents. A major health concern is that these women will continue to use MAP during pregnancy. The purpose of this study was to investigate whether MAP administered to the mother during pregnancy would change the expression of α- and β- myosin heavy chain (MHC) mRNA in rat neonatal hearts, as detected by quantitative RT-PCR. In addition, morphological changes in the rat neonatal ventricles were examined. Pregnant rats were injected intraperitoneally with MAP (1 mg/kg/day) starting at day 0 of gestation and ending at day 21. There was a significant increase in α-MHC mRNA expression in the neonatal ventricular muscle in the experimental group compared with the control at postnatal day (P) 0 and 5. α-MHC mRNA expression in both groups was similar after P9. β-MHC mRNA expression was similar in both groups at P0. Postnatal β-MHC mRNA expression decreased rapidly, but significant alteration was not detected. Neonatal rats at P0 exhibited some cardiac changes, including hypertrophy, degeneration, and disarrangement of myofibers, but these lesions disappeared by P14. We conclude that chronic maternal administration of MAP changes the α- and β-MHC mRNA expression pattern in fetal and neonatal hearts, correlating with abnormal development, plasma level of hormones, and myocardial damage. At the same time, it is indicated that neonatal cardiomyocytes have reversibility.

Original languageEnglish
Pages (from-to)1529-1540
Number of pages12
JournalLife Sciences
Issue number12
Publication statusPublished - 2004 Feb 6


  • Fetal heart
  • Hormone
  • Methamphetamine
  • Pregnancy
  • Quantitative RT-PCR
  • mRNA
  • α-MHC
  • β-MHC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)


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