Measurement of interleukin-4 and histamine in superficial cells of conjunctiva in patients with allergic conjunctivitis

Hiroshi Fujishima, Ichiro Saito, Tsutomu Takeuchi, Naoshi Shinozaki, Kazuo Tsubota

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Interleukin 4 (IL-4) may play a role in the development of allergic disease. We questioned whether IL-4 related phenomenon occurred on the surface of the conjunctiva during allergic conjunctivitis. Ten patients with cedar pollen-allergic conjunctivitis and 10 patients with postsurgical conjunctivitis were enrolled in this study. Cells were collected by brush cytology from the upper palpebral conjunctiva. After the cells were cultured for 24 hours, the levels of IL-4, IgE, and histamine were measured in the supernatants. An ELISA was used for histamine and a highly sensitive sandwich ELISA for IL-4 and IgE. Conjunctival cells were also stained and fixed by May-Grunwalds stain solution. The number of superficial cells, especially lymphocytes, were compared in the two groups. The total number of cells collected by brush cytology did not differ in allergic vs., postsurgical conjunctivitis. Lymphocytes were similarly present in such specimens. The levels of IL-4 (p = 0.01) and histamine (p = 0.02) significantly increased in the specimens from patients with allergic conjunctivitis, although IgE was not detected in both groups. There was a correlation between the level of IL-4 and histamine (p = 0.0001). Conjunctival cells in allergic conjunctivitis produced a larger amount of IL-4 and histamine for cedar pollen compared to the postsurgical conjunctivitis. As with other allergic diseases, IL-4 may play a role similar to that of histamine in the development of allergic conjunctivitis.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalCurrent Eye Research
Issue number2
Publication statusPublished - 1996 Feb
Externally publishedYes


  • Allergic conjunctivitis
  • Conjunctival cells
  • Histamine
  • Human
  • Interleukin-4

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Measurement of interleukin-4 and histamine in superficial cells of conjunctiva in patients with allergic conjunctivitis'. Together they form a unique fingerprint.

Cite this