Measurement of urinary mesotocin in large-billed crows by enzyme-linked immunosorbent assay

Akiko Seguchi, Kazutaka Mogi, Ei Ichi Izawa

Research output: Contribution to journalComment/debatepeer-review

2 Citations (Scopus)


Mesotocin (MT) is an avian homologue of oxytocin (OT). Behavioral pharmacological studies in birds have suggested the involvement of MT in socially affiliative behavior. However, investigations of peripheral MT levels associated with social behavior are lacking because non-invasive methods to measure surrogate plasma MT have yet to be established. This study aimed to measure urinary MT in crows using a commercially available OT enzyme-linked immunosorbent assay kit. Urine samples were collected after intravenous injection of MT and centrifuged to separate urine and fecal components. We found that urinary MT was significantly elevated 15–30 min after MT injection. These results validate our method for the use of urine samples for the measurement of peripheral MT levels in crows.

Original languageEnglish
Pages (from-to)520-524
Number of pages5
JournalJournal of Veterinary Medical Science
Issue number4
Publication statusPublished - 2022


  • avian
  • corvid
  • enzyme-linked immunoassay
  • mesotocin
  • oxytocin

ASJC Scopus subject areas

  • General Veterinary


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