Measurements of the equivalent whole-body dose during radiation therapy by cytogenetic methods

Marco Durante, Shigeru Yamada, Koichi Ando, Yoshiya Furusawa, Tetsuya Kawata, Hideyuki Majima, Takashi Nakano, Hirohiko Tsujii

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Estimates of equivalent whole-body dose following partial body exposure can be performed using different biophysical models. Calculations should be compared with biodosimetry data, but measurements are complicated by mitotic selection induced in target cells after localized irradiation. In this paper we measured chromosomal aberrations in peripheral blood lymphocytes during radiotherapy, and estimated the equivalent whole-body dose absorbed, by using the novel technique of interphase chromosome painting. Premature chromosome condensation was induced in stimulated lymphocytes by incubation in calyculin A, and slides were hybridized in situ with whole-chromosome DNA probes specific for human chromosomes 2 and 4. Reciprocal exchanges were used to estimate the equivalent whole-body dose, based on individual pre-treatment in vitro calibration curves. Equivalent whole-body dose increased as a function of the number of fractions, and reached a plateau at high fraction numbers. Chromosomal aberration yields were dependent on field size, tumour position and concurrent chemotherapy. Results suggest that interphase chromosome painting is a simple technique able to give a reliable estimate of the equivalent whole-body dose absorbed during therapeutic partial-body irradiation.

Original languageEnglish
Pages (from-to)1289-1298
Number of pages10
JournalPhysics in Medicine and Biology
Issue number5
Publication statusPublished - 1999 May 1

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging


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