Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation

Aya Mizutani Akimoto, Erika Hasuike Niitsu, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

Original languageEnglish
Article number1253
JournalInternational journal of molecular sciences
Issue number4
Publication statusPublished - 2018 Apr 21


  • Cell culture
  • Hydrogel
  • Mesenchymal stem cell
  • mechanobiology
  • poly(N-isopropylacrylamide)

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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