Metabolome analysis based on capillary electrophoresis-mass spectrometry

Akiyoshi Hirayama, Masataka Wakayama, Tomoyoshi Soga

Research output: Contribution to journalReview articlepeer-review

76 Citations (Scopus)


Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalTrAC - Trends in Analytical Chemistry
Publication statusPublished - 2014 Oct 1


  • CE-MS
  • Capillary electrophoresis
  • Energy metabolism
  • Mass spectrometry
  • Metabolite identification
  • Metabolome analysis
  • Metabolomics
  • Sample pretreatment
  • Separation
  • Sheathless interface

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy


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