Metabolomics Analysis of Blood, Urine, and Saliva Samples Based on Capillary Electrophoresis–Mass Spectrometry

Masahiro Sugimoto, Yumi Aizawa

Research output: Chapter in Book/Report/Conference proceedingChapter


Capillary electrophoresis–mass spectrometry (CE–MS) is an ideal method for analyzing various metabolites in biological samples. CE–MS can simultaneously identify and quantify hundreds of charged metabolites using only two acquisition methods for positively and negatively charged metabolites. Furthermore, CE–MS is commonly used for analyzing biological samples to understand the pathology of diseases at the metabolic level and biofluid samples, such as blood and urine, to explore biomarkers. Here, we introduce a protocol that delineates the handling of clinical samples to ensure that the CE–MS analysis yields reproducible quantified data. We have focused on sample collection, storage, processing, and measurement. Although the implementation of rigorous standard operating protocols is preferred for enhancing the quality of the samples, various limitations in an actual clinical setting make it difficult to adhere to strict rules. Therefore, the effect of each process on the quantified metabolites needs to be evaluated to design a protocol with acceptable tolerances. Furthermore, quality controls and assessments to handle clinical samples are introduced.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Biofluid
  • Capillary electrophoresis–mass spectrometry
  • Clinical samples

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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