Microfluidic experimental platform for producing size-controlled three-dimensional spheroids

Hiroki Ota, Norihisa Miki

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells were aggregated into chambers arranged in an array by microrotational flow within 120 s to form spheroids. The cell density of the initial medium and hydrodynamic flow in the developed array could be adjusted while keeping the device geometry the same to control spheroid size with a standard deviation of less than 19% of the mean. Using this device, spheroids of HepG2 cells of various size categories could be maintained for three days in the chamber with medium exchange and could be continuously evaluated for topology and hepatic functions. Furthermore, CYP1A1 activities were found to increase with time to a constant level at three days. These results demonstrate that this device is readily applicable to producing and maintaining spheroids for in vitro drug screening and biological research.

Original languageEnglish
Pages (from-to)266-273
Number of pages8
JournalSensors and Actuators, A: Physical
Issue number2
Publication statusPublished - 2011 Oct


  • Bio MEMS
  • Microfluidics
  • Microrotational flow
  • Spheroid

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering


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