Microfluidic experimental platform using micro-rotation flow for producing multiple size-controlled three-dimensional spheroids

Hiroki Ota, Taiga Kodama, Norihisa Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells are collected to form a spheroid in chambers by micro-rotational flow in two minutes. The developed array could control the size of three-dimensional spheroids hydrodynamically with the standard deviations less than 19 % by varying the cell density of the medium without altering the device geometry. Using the developed perfusion system we experimentally observed that the sizes of formed spheroids remained constant for two days and that the detoxification enzyme, CYP1A1, activities increased.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages2080-2082
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period10/10/310/10/7

Keywords

  • Hepatocyte
  • Micro-rotation
  • Microfluidics
  • Spheroid

ASJC Scopus subject areas

  • Control and Systems Engineering

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