TY - JOUR
T1 - Microglia-specific localisation of a novel calcium binding protein, Iba1
AU - Ito, Daisuke
AU - Imai, Yoshinori
AU - Ohsawa, Keiko
AU - Nakajima, Kazuyuki
AU - Fukuuchi, Yasuo
AU - Kohsaka, Shinichi
N1 - Funding Information:
This study was supported by Grants from the Japanese Ministry of Health and Welfare, the Science and Technology Agency, Japan, and by a Grant-in-Aid for Scientific Research on Priority Areas from the Japanese Ministry of Education, Science, Sports and Culture.
PY - 1998/6/1
Y1 - 1998/6/1
N2 - Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain: respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo. and that Tba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.
AB - Recently it has been shown that mRNA of Iba1 (ionized calcium binding adaptor molecule 1), which was a novel calcium binding protein cDNA-cloned by our group, is specifically expressed in microglia in cultures of rat brain cells. In the present study, immunocytochemical and immunohistochemical examinations demonstrated that Iba1 protein is expressed in microglia alone both in cultured brain cells and in the brain: respectively. In a mixed cell culture of embryonic rat brain, immunocytochemically positive for Iba1 protein were the microglia but it was not detectable in neurons, astroglia, or oligodendroglia. Immunohistochemical staining of adult rat brain sections showed Iba1 protein to be specifically localised in ramified microglia. In addition, immunohistochemical staining and immunoblot analysis of activated microglia in the facial nucleus after facial nerve axotomy shows that expression of Iba1 protein was upregulated and peaked at 7 days. These results indicated that localisation of Iba1 protein is restricted to microglia both in vitro and in vivo. and that Tba1 protein plays a role in regulating the function of microglia, especially in the activated microglia.
KW - Calcium binding protein
KW - Facial nerve axotomy
KW - Iba1
KW - Immunocytochemistry
KW - Immunohistochemistry
KW - Microglia
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U2 - 10.1016/S0169-328X(98)00040-0
DO - 10.1016/S0169-328X(98)00040-0
M3 - Article
C2 - 9630473
AN - SCOPUS:0032102358
SN - 0169-328X
VL - 57
SP - 1
EP - 9
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -