Micropatterning of bacterial cellulose as degradable substrate for cell culture

Yuya Karita; Kayoko Hirayama; Hiroaki Onoe; Shoji Takeuchi

doi:10.1109/MEMSYS.2014.6765691

Micropatterning of bacterial cellulose as degradable substrate for cell culture

Yuya Karita, Kayoko Hirayama, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This paper describes microfabrication of bacterial cellulose membrane. Bacterial cellulose is a nanofibrous cellulosic material produced by the bacteria called Acetobacter xylinum. We micropatterned a bacterial cellulose membrane by utilizing MEMS process. This patterned bacterial cellulose worked as a scaffold for mouse embryonic fibroblast cells: The cells attached and grew on the patterned bacterial cellulose membrane. Moreover, formation of cell cluster was observed by the treatment of cellulose degrading enzyme. We believe that this micropatterned cellulose membrane would be useful as degradable microscaffolds for cell culture.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	518-519
Number of pages	2
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765691
Publication status	Published - 2014
Externally published	Yes
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States Duration: 2014 Jan 26 → 2014 Jan 30

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/Territory	United States
City	San Francisco, CA
Period	14/1/26 → 14/1/30

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2014.6765691

Cite this

Karita, Y., Hirayama, K., Onoe, H., & Takeuchi, S. (2014). Micropatterning of bacterial cellulose as degradable substrate for cell culture. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 518-519). Article 6765691 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765691

Micropatterning of bacterial cellulose as degradable substrate for cell culture. / Karita, Yuya; Hirayama, Kayoko; Onoe, Hiroaki et al.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 518-519 6765691 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Karita, Y, Hirayama, K, Onoe, H & Takeuchi, S 2014, Micropatterning of bacterial cellulose as degradable substrate for cell culture. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765691, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 518-519, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 14/1/26. https://doi.org/10.1109/MEMSYS.2014.6765691

Karita Y, Hirayama K, Onoe H, Takeuchi S. Micropatterning of bacterial cellulose as degradable substrate for cell culture. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 518-519. 6765691. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2014.6765691

Karita, Yuya ; Hirayama, Kayoko ; Onoe, Hiroaki et al. / Micropatterning of bacterial cellulose as degradable substrate for cell culture. MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 518-519 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "This paper describes microfabrication of bacterial cellulose membrane. Bacterial cellulose is a nanofibrous cellulosic material produced by the bacteria called Acetobacter xylinum. We micropatterned a bacterial cellulose membrane by utilizing MEMS process. This patterned bacterial cellulose worked as a scaffold for mouse embryonic fibroblast cells: The cells attached and grew on the patterned bacterial cellulose membrane. Moreover, formation of cell cluster was observed by the treatment of cellulose degrading enzyme. We believe that this micropatterned cellulose membrane would be useful as degradable microscaffolds for cell culture.",

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AB - This paper describes microfabrication of bacterial cellulose membrane. Bacterial cellulose is a nanofibrous cellulosic material produced by the bacteria called Acetobacter xylinum. We micropatterned a bacterial cellulose membrane by utilizing MEMS process. This patterned bacterial cellulose worked as a scaffold for mouse embryonic fibroblast cells: The cells attached and grew on the patterned bacterial cellulose membrane. Moreover, formation of cell cluster was observed by the treatment of cellulose degrading enzyme. We believe that this micropatterned cellulose membrane would be useful as degradable microscaffolds for cell culture.

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Micropatterning of bacterial cellulose as degradable substrate for cell culture

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